Combined heterozygosity for methylenetetrahydrofolate reductase (MTHFR) mutations C677T and A1298C is associated with abruptio placentae but not with intrauterine growth restriction
Date
2001, 2001
Authors
Gebhardt G.S.
Scholtz C.L.
Hillermann R.
Odendaal H.J.
Gebhardt G.S.
Scholtz C.L.
Hillermann R.
Odendaal H.J.
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Objective: This study was undertaken to investigate the involvement of MTHFR gene mutations C677T and A1298C implicated in vascular disease, in patients with abruptio placentae and intrauterine growth restriction (IUGR). Study Design: DNA was extracted from blood samples of 54 patients with placental vasculopathy (18 patients with abruptio placentae and 36 with IUGR) and 114 control patients and amplified by the polymerase chain reaction (PCR). The resulting fragments were subjected to restriction enzyme analysis and resolved by gel electrophoresis. Results: A significant association could be demonstrated between mutation A1298C and both abruptio placentae and IUGR. Combined heterozygosity for mutations C677T and A1298C was detected in 22.2% of abruptio placentae cases. Conclusions: Combined heterozygosity for MTHFR mutations C677T and A1298C may represent a genetic marker for abruptio placentae. Copyright © 2001 Elsevier Science Ireland Ltd.
Objective: This study was undertaken to investigate the involvement of MTHFR gene mutations C677T and A1298C implicated in vascular disease, in patients with abruptio placentae and intrauterine growth restriction (IUGR). Study Design: DNA was extracted from blood samples of 54 patients with placental vasculopathy (18 patients with abruptio placentae and 36 with IUGR) and 114 control patients and amplified by the polymerase chain reaction (PCR). The resulting fragments were subjected to restriction enzyme analysis and resolved by gel electrophoresis. Results: A significant association could be demonstrated between mutation A1298C and both abruptio placentae and IUGR. Combined heterozygosity for mutations C677T and A1298C was detected in 22.2% of abruptio placentae cases. Conclusions: Combined heterozygosity for MTHFR mutations C677T and A1298C may represent a genetic marker for abruptio placentae. Copyright © 2001 Elsevier Science Ireland Ltd.
Objective: This study was undertaken to investigate the involvement of MTHFR gene mutations C677T and A1298C implicated in vascular disease, in patients with abruptio placentae and intrauterine growth restriction (IUGR). Study Design: DNA was extracted from blood samples of 54 patients with placental vasculopathy (18 patients with abruptio placentae and 36 with IUGR) and 114 control patients and amplified by the polymerase chain reaction (PCR). The resulting fragments were subjected to restriction enzyme analysis and resolved by gel electrophoresis. Results: A significant association could be demonstrated between mutation A1298C and both abruptio placentae and IUGR. Combined heterozygosity for mutations C677T and A1298C was detected in 22.2% of abruptio placentae cases. Conclusions: Combined heterozygosity for MTHFR mutations C677T and A1298C may represent a genetic marker for abruptio placentae. Copyright © 2001 Elsevier Science Ireland Ltd.
Description
Keywords
adult; allele; article; controlled study; DNA determination; female; gel electrophoresis; gene mutation; genotype; heterozygosity; human; human tissue; intrauterine growth retardation; major clinical study; polymerase chain reaction; priority journal; restriction mapping; solutio placentae; vascular disease; Abruptio Placentae; DNA Mutational Analysis; Female; Fetal Growth Retardation; Gene Frequency; Heterozygote; Humans; Methylenetetrahydrofolate Reductase (NADPH2); Mutation; Oxidoreductases Acting on CH-NH Group Donors; Placenta; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Pregnancy; Vascular Diseases, adult, allele, article, controlled study, DNA determination, female, gel electrophoresis, gene mutation, genotype, heterozygosity, human, human tissue, intrauterine growth retardation, major clinical study, polymerase chain reaction, priority journal, restriction mapping, solutio placentae, vascular disease, Abruptio Placentae, DNA Mutational Analysis, Female, Fetal Growth Retardation, Gene Frequency, Heterozygote, Humans, Methylenetetrahydrofolate Reductase (NADPH2), Mutation, Oxidoreductases Acting on CH-NH Group Donors, Placenta, Polymerase Chain Reaction, Polymorphism, Restriction Fragment Length, Pregnancy, Vascular Diseases
Citation
European Journal of Obstetrics Gynecology and Reproductive Biology
97
2
European Journal of Obstetrics Gynecology and Reproductive Biology
97
2
97
2
European Journal of Obstetrics Gynecology and Reproductive Biology
97
2