Combined heterozygosity for methylenetetrahydrofolate reductase (MTHFR) mutations C677T and A1298C is associated with abruptio placentae but not with intrauterine growth restriction

Simple item page
dc.contributor.authorGebhardt G.S.
dc.contributor.authorScholtz C.L.
dc.contributor.authorHillermann R.
dc.contributor.authorOdendaal H.J.
dc.contributor.authorGebhardt G.S.
dc.contributor.authorScholtz C.L.
dc.contributor.authorHillermann R.
dc.contributor.authorOdendaal H.J.
dc.date.accessioned2011-05-15T16:16:21Z
dc.date.accessioned2011-05-15T16:16:21Z
dc.date.available2011-05-15T16:16:21Z
dc.date.available2011-05-15T16:16:21Z
dc.date.issued2001
dc.date.issued2001
dc.description.abstractObjective: This study was undertaken to investigate the involvement of MTHFR gene mutations C677T and A1298C implicated in vascular disease, in patients with abruptio placentae and intrauterine growth restriction (IUGR). Study Design: DNA was extracted from blood samples of 54 patients with placental vasculopathy (18 patients with abruptio placentae and 36 with IUGR) and 114 control patients and amplified by the polymerase chain reaction (PCR). The resulting fragments were subjected to restriction enzyme analysis and resolved by gel electrophoresis. Results: A significant association could be demonstrated between mutation A1298C and both abruptio placentae and IUGR. Combined heterozygosity for mutations C677T and A1298C was detected in 22.2% of abruptio placentae cases. Conclusions: Combined heterozygosity for MTHFR mutations C677T and A1298C may represent a genetic marker for abruptio placentae. Copyright © 2001 Elsevier Science Ireland Ltd.
dc.description.abstractObjective: This study was undertaken to investigate the involvement of MTHFR gene mutations C677T and A1298C implicated in vascular disease, in patients with abruptio placentae and intrauterine growth restriction (IUGR). Study Design: DNA was extracted from blood samples of 54 patients with placental vasculopathy (18 patients with abruptio placentae and 36 with IUGR) and 114 control patients and amplified by the polymerase chain reaction (PCR). The resulting fragments were subjected to restriction enzyme analysis and resolved by gel electrophoresis. Results: A significant association could be demonstrated between mutation A1298C and both abruptio placentae and IUGR. Combined heterozygosity for mutations C677T and A1298C was detected in 22.2% of abruptio placentae cases. Conclusions: Combined heterozygosity for MTHFR mutations C677T and A1298C may represent a genetic marker for abruptio placentae. Copyright © 2001 Elsevier Science Ireland Ltd.
dc.description.versionArticle
dc.description.versionArticle
dc.identifier.citationEuropean Journal of Obstetrics Gynecology and Reproductive Biology
dc.identifier.citation97
dc.identifier.citation2
dc.identifier.citationEuropean Journal of Obstetrics Gynecology and Reproductive Biology
dc.identifier.citation97
dc.identifier.citation2
dc.identifier.issn03012115
dc.identifier.issn03012115
dc.identifier.other10.1016/S0301-2115(00)00540-6
dc.identifier.other10.1016/S0301-2115(00)00540-6
dc.identifier.urihttp://hdl.handle.net/10019.1/13745
dc.identifier.urihttp://hdl.handle.net/10019.1/13745
dc.subjectadult; allele; article; controlled study; DNA determination; female; gel electrophoresis; gene mutation; genotype; heterozygosity; human; human tissue; intrauterine growth retardation; major clinical study; polymerase chain reaction; priority journal; restriction mapping; solutio placentae; vascular disease; Abruptio Placentae; DNA Mutational Analysis; Female; Fetal Growth Retardation; Gene Frequency; Heterozygote; Humans; Methylenetetrahydrofolate Reductase (NADPH2); Mutation; Oxidoreductases Acting on CH-NH Group Donors; Placenta; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Pregnancy; Vascular Diseases
dc.subjectadult
dc.subjectallele
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectDNA determination
dc.subjectfemale
dc.subjectgel electrophoresis
dc.subjectgene mutation
dc.subjectgenotype
dc.subjectheterozygosity
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectintrauterine growth retardation
dc.subjectmajor clinical study
dc.subjectpolymerase chain reaction
dc.subjectpriority journal
dc.subjectrestriction mapping
dc.subjectsolutio placentae
dc.subjectvascular disease
dc.subjectAbruptio Placentae
dc.subjectDNA Mutational Analysis
dc.subjectFemale
dc.subjectFetal Growth Retardation
dc.subjectGene Frequency
dc.subjectHeterozygote
dc.subjectHumans
dc.subjectMethylenetetrahydrofolate Reductase (NADPH2)
dc.subjectMutation
dc.subjectOxidoreductases Acting on CH-NH Group Donors
dc.subjectPlacenta
dc.subjectPolymerase Chain Reaction
dc.subjectPolymorphism, Restriction Fragment Length
dc.subjectPregnancy
dc.subjectVascular Diseases
dc.titleCombined heterozygosity for methylenetetrahydrofolate reductase (MTHFR) mutations C677T and A1298C is associated with abruptio placentae but not with intrauterine growth restriction
dc.titleCombined heterozygosity for methylenetetrahydrofolate reductase (MTHFR) mutations C677T and A1298C is associated with abruptio placentae but not with intrauterine growth restriction
dc.typeArticle
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications