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Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4

dc.contributor.authorAguayo, Jaimeen_ZA
dc.contributor.authorMostert, Dianeen_ZA
dc.contributor.authorFourrier-Jeandel, Celineen_ZA
dc.contributor.authorCerf-Wendling, Isabelleen_ZA
dc.contributor.authorHostachy, Brunoen_ZA
dc.contributor.authorViljoen, Altusen_ZA
dc.contributor.authorIoos, Renauden_ZA
dc.date.accessioned2017-03-15T09:49:48Z
dc.date.available2017-03-15T09:49:48Z
dc.date.issued2017-02-08
dc.identifier.citationAguayo, J. et al. 2017. Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4. PLoS ONE, 12(2):e0171767, doi:10.1371/journal.pone.0171767.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0171767
dc.identifier.urihttp://hdl.handle.net/10019.1/100680
dc.descriptionCITATION: Aguayo, J. et al. 2017. Development of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4. PLoS ONE, 12(2):e0171767, doi:10.1371/journal.pone.0171767.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractFusarium oxysporum f. sp. cubense (Foc) is one of the most important threats to global banana production. Strategies to control the pathogen are lacking, with plant resistance offering the only long-term solution, if sources of resistance are available. Prevention of introduction of Foc into disease-free areas thus remains a key strategy to continue sustainable banana production. In recent years, strains of Foc affecting Cavendish bananas have destroyed plantations in a number of countries in Asia and in the Middle East, and one African country. One vegetative compatibility group (VCG), 01213/16, is considered the major threat to bananas in tropical and subtropical climatic conditions. However, other genetically related VCGs, such as 0121, may potentially jeopardize banana cultures if they were introduced into disease-free areas. To prevent the introduction of these VCGs into disease-free Cavendish banana-growing countries, a real-time PCR test was developed to accurately detect both VCGs. A previously described putative virulence gene was used to develop a specific combination of hydrolysis probe/primers for the detection of tropical Foc race 4 strains. The real-time PCR parameters were optimized by following a statistical approach relying on orthogonal arrays and the Taguchi method in an attempt to enhance sensitivity and ensure high specificity of the assay. This study also assessed critical performance criteria, such as repeatability, reproducibility, robustness, and specificity, with a large including set of 136 F. oxysporum isolates, including 73 Foc pathogenic strains representing 24 VCGs. The validation data demonstrated that the new assay could be used for regulatory testing applications on banana plant material and can contribute to preventing the introduction and spread of Foc strains affecting Cavendish bananas in the tropics.en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0171767
dc.format.extent20 pages
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.subjectFusarium oxysporumen_ZA
dc.subjectBananasen_ZA
dc.titleDevelopment of a hydrolysis probe-based real-time assay for the detection of tropical strains of Fusarium oxysporum f. sp. cubense race 4en_ZA
dc.typeArticleen_ZA
dc.description.versionPublisher's version
dc.rights.holderAuthors retain copyright


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