Browsing by Author "Swart, Liezel Maria"

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    The biochemical study of the R- and S-enantiomers of 2-(4-acetoxyphenyl)-2-chloro-Nmethylethylammonium chloride (Compound A).
    (Stellenbosch : Stellenbosch University, 2017-03) Swart, Liezel Maria; Swart, Pieter; Swart, Amanda C.; Stellenbosch University. Faculty of Science. Dept. of Biochemistry.
    ENGLISH ABSTRACT: The study describes: . The synthesis of a racemic mixture of Compound A (2-(4-acetoxyphenyl)-2-chloro-N-methylethylammonium chloride), a synthetic analogue of the active compound isolated from the African shrub, Salsola tuberculataformis Botschantzev. . The development of a strategy to separate the R- and S-enantiomers of Compound A. . The isolation and purification of a highly purified substrate-free cytochrome P450 11β-hydroxylase (CYP11B1) from ovine adrenals. . The isolation of a partially purified mixture of the mitochondrial electron transport chain, adrenodoxin reductase and adrenodoxin from ovine adrenals. . The investigation into the mechanism of action of the R- and S-enantiomers of Compound A on the spectroscopic properties of substrate-free CYP11B1. . The investigation into the influence of the R- and S-enantiomers of Compound A on the mitochondrial electron transport chain by using cytochrome c as an electron acceptor.