Stellenbosch University - Scopus Tygerberg Hospital Publications

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Browsing Stellenbosch University - Scopus Tygerberg Hospital Publications by Author "Abayomi, A."

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    Incidence of Haemolytic Disease of the Newborn in Harare, Zimbabwe
    (2008) Mandisodza, A. R.; Mangoyi, G.; Musekiwa, Z.; Mvere, D.; Abayomi, A.
    Background: Haemolytic Disease of Newborn is responsible for a number of neonatal deaths and complications worldwide. Its incidence in Zimbabwe is not clearly understood. Objective: To find out incidence of Haemolytic Disease of Newborn in Harare and ability to identify antibodies associated with HDN. Methods: A retrospective cross sectional study at the Parirenyatwa Group of Hospitals and National Blood Service, Zimbabwe (NBSZ). We studied 22493 infants at Parirenyatwa Hospital during the 1995-1997 and 2002-2003 periods. The main outcome measures were ABO and Rh blood group results, Direct Antiglobulin Test and Maternal Antibody Screening and Identification Results were obtained from the NBSZ. Results: One hundred and ninety-one (0,85%) infants had Haemolytic Disease of Newborn. One hundred and sixty-three (85.34 %) of these were due to anti-AB, twenty five (13.09%) were due to anti-D and three (1.57%) due to anti-Kell. Incidence of Haemolytic Disease of Newborn during 1995-1997 and 2002-2003, were 0.93% and 0.64 %, respectively (p = 0.014). ABO Haemolytic Disease of Newborn showed a statistically significant difference during the two periods (p=0.003). Rhesus Haemolytic Disease of Newborn showed no statistically significant difference during same periods (p=0.317). Conclusion: The incidence of Haemolytic Disease of Newborn at Parirenyatwa Hospital is comparable with that found in other countries. It is recommended that K antigen be included in the pretransfusion testing to avoid anti-K related Haemolytic Disease of Newborn.
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    Performance of the panleucogating protocol for CD4+ T cell enumeration in an HIV dedicated laboratory facility in Barbados
    (2008) Sippy-Chatrani, N.; Marshall, S.; Branch, S.; Carmichael-Simmons, K.; Landis, R. C.; Abayomi, A.
    Objective: To compare the Panleucogating (PLG) protocol with the routinely used four-color protocol for CD4+ T cell count enumeration. Design and Methods: One hundred fifty-three blood samples were randomly selected from samples received at the National HIV Laboratory for routine immunological monitoring. Samples were prepared using Coulter CYTO-STAT® tetraCHROME monoclonal antibodies and FlowCARE™ PLG CD4 reagent for four-color and PLG, respectively, and analyzed on the Beckman Coulter EPICS XL flow cytometer. The PLG protocol used a sequential gating strategy where CD4+ T cells were identified using side scatter properties of cells and CD45 staining. The four-color protocol used CD45 and CD3 to identify CD4+ T cells. Results: Absolute CD4+ T cell counts and percentages ranged from 4 to 1,285 cells/μL and 0.9 to 46.7%, respectively. Linear regression analyses revealed good correlation of PLG with the four-color protocol (absolute counts, R2 = 0.95; percentages, R2 = 0.98) over the entire range including the clinically relevant range. Bland Altman statistics revealed no bias for CD4 counts <500 cells/μL and a slight underestimation by PLG for counts >500 cells/μL (Bias = -32.7 cells/μL; 95% agreement limits = -151.3- +86.0). CD4+ T cell percentages were the similar over the entire range (Bias = 0.6%; 95% agreement limits = -1.97 ± 3.18). Conclusions: PLG is an accurate method for enumerating CD4+ T cells and has resulted in major cost savings to the Government of Barbados. This has implications for the sustainability of the National HIV containment program in Barbados and the other resource limited Caribbean countries. The PLG technique is now being routinely used in Barbados. © 2008 Clinical Cytometry Society.
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    The human immunodeficiency virus epidemic: A race against time for millions and the role of flow cytometry. A Caribbean and resource-constrained country perspective
    (2007) Abayomi, A.
    There is a race against time for millions in the world today. Both the technology and the manpower are currently available to deliver the services that are required to meet the needs of the 40 million people currently living with HIV/AIDS, but at what price? The reality is therefore that we are a long shot away from this realization. What are the facts and why have we not achieved even the simplest deadlines set by the World Health Organization (WHO)? Are these objectives realistic? What role does hard science have to play in the search for cost-effective solutions and futuristic effective options? To stem this unrelenting epidemic and convert the natural history of the disorder in those already living with the virus into one of chronicity, rather than one characterized by a dehumanizing and stigmatized death, requires a global commitment at all levels. This discussion examines the reality and offers a snapshot of capacity and experiences in the developing world. Crucially it looks at the immediate and long term role of flow cytometry in the expanded care and treatment programs for developing nations. © 2007 Clinical Cytometry Society.
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    Wireless networks for surveillance, data capture and data management in the human immunodeficiency virus epidemic care and treatment programmes.
    (2006) Abayomi, A.; Goodridge, W.; Asika, O.
    Biomedical and demographic data capture and the subsequent management of such information are critical factors in the implementation of any level of healthcare prevention and treatment program. The developing world is seriously handicapped by lack of infrastructure to acquire such data let alone manipulate the information banks for projections, forecasting and priority project planning. With this in mind we set about to use the recent proliferation of wireless cellular networks and easily accessible Personal Digital Assistants (PDA), to devise a means of collecting such data even from the most remote primary healthcare facility. Our priority is aimed at initially at providing a support technology for the HIV expanded program. This technology can be implemented in the absence of computerization and regular power supply. Utilizing a PDA to capture patient data (demographic, clinical and laboratory parameters), the healthcare giver can use a wireless link between the PDA and a cellular phone to transfer the data to a central medical data base. These can then become permanent and secure data banks for future use by health providers, either at the same location or at other health facility that have authorized access to the data bank. It also affords a platform for integrating reference labs into the network as well as the opportunity to disseminate continuing medical educational material. The network can also be adapted to electronic remote consultations and eventually its data banks can be assimilated into protocols of artificial intelligence and data mining.