(Stellenbosch : Stellenbosch University, 1993-03) Nackerdien, Zeena Elnisa; Bohm, Lothar; Dizdaroglu, M.; Stellenbosch University. Faculty of Medicine and Health Sciences. Department of Biomedical Sciences: Molecular Biology and Human Genetics
ENGLISH ABSTRACT: Oxygen-derived free radicals are implicated in many human
diseases including cancer. The mutagenic and carcinogenic
properties of these species manifest themselves in lingering
damage to several biomolecules including DNA. Here, efforts
have been made to chemically characterize DNA base damage and
DNA-protein cross-links produced in isolated chromatin and in
cultured mammalian cells exposed to ionizing radiation or to
H2O2/metal ions. Gas chromatography/mass spectrometry with
selected ion-monitoring was used for this purpose.