Factors involved in the regulation of the Schizosaccharomyces pombe malic enzyme gene
Date
2001
Authors
Groenewald M.
Viljoen-Bloom M.
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Abstract
Transcription of the Schizosaccharomyces pombe malic enzyme gene, mae2, is induced when cells are grown on high glucose concentrations or under non-aerated conditions. Two cis-acting elements in the mae2 promoter, upstream activator sequences UAS1 and UAS2, are required for basal expression, whilst three negative-acting, upstream repressor sequences are involved in general derepression of mae2. Both the Pka1 and Sty1 signal transduction pathways are involved in the induced expression of mae2 under fermentative conditions. Expression of mae2 seems to be regulated in response to the carbon source, lack of oxygen and osmotic stress conditions, probably to assist in maintaining the intracellular redox balance.
Description
Keywords
carbon, cis acting element, glucose, malate dehydrogenase (decarboxylating), oxygen, protein kinase, aeration, article, cell growth, controlled study, culture medium, fermentation, gene expression regulation, gene repression, gene sequence, genetic regulation, genetic transcription, nonhuman, osmotic stress, oxidation reduction state, oxygen supply, priority journal, promoter region, Schizosaccharomyces pombe, signal transduction, Amidohydrolases, Bacterial Proteins, Base Sequence, Binding Sites, DNA Mutational Analysis, Fermentation, Gene Expression Regulation, Enzymologic, Glucose, Maleates, Molecular Sequence Data, Oxygen, Promoter Regions (Genetics), RNA, Schizosaccharomyces, Sequence Analysis, DNA, Signal Transduction, Transcription Factors, Transcription, Genetic, Schizosaccharomyces pombe
Citation
Current Genetics
39
4
39
4