Potential of South African entomopathogenic nematodes to control the leaf miner, Holocacista capensis (Lepidoptera: Heliozelidae)

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dc.contributor.authorSteyn, L. A. I.en_ZA
dc.contributor.authorAddison, P.en_ZA
dc.contributor.authorMalan, A. P.en_ZA
dc.date.accessioned2021-11-12T13:39:43Z
dc.date.available2021-11-12T13:39:43Z
dc.date.issued2019
dc.descriptionCITATION: Steyn, L. A. I., Addison, P. & Malan, A. P. 2019. Potential of South African entomopathogenic nematodes to control the leaf miner, Holocacista capensis (Lepidoptera: Heliozelidae). South African Journal of Enology and Viticulture, 40(2):301-309. doi:10.21548/40-2-3420
dc.descriptionThe original publication is available at https://www.journals.ac.za/index.php/sajev/index
dc.description.abstractThe Cape grapevine leafminer, Holocacista capensis, a sporadic pest of economic importance, is found in South African table and wine grape vineyards. The cocoon casings, constructed by the final instar larvae, can be found attached to grape bunches, posing a phytosanitary risk for table grape export markets. The current study aimed to determine the susceptibility of leaf-mining H. capensis larvae toseven entomopathogenic nematode (EPN) species belonging to Steinernematidae and Heterorhabditidae. To determine nematode virulence occupied leaf-mining galleries were extracted from infested leaves and inoculated with 200 infective juveniles (IJs) in 50 μl of distilled water, for each of the EPNs screened. Concentration assays were conducted and and lethal dose was determined for the three most virulent species (Heterorhabditis baujardi, H. indica and H. noenieputensis), using concentrations of 0, 25, 50, 100, 200, and 400 IJs/leaf-mining larva. High mortality of leaf-mining larvae was obtained with H. baujardi(92%), H. noenieputensis (85%) and H. indica (83%). Almost double the number of H. noenieputensis (34 nematodes/insect) penetrated the insect larvae, in comparison with the other two EPNs. However, the relative potency of H. baujardi was 3.56 times higher than for H. indica, whilst that of H. indica was 2.57times higher than it was for H. noenieputensis. The results obtained in the laboratory were found to be encouraging, especially with regard to the nematodes’ ability to penetrate the leaf-mining galleries, and to infect the larvae successfully.en_ZA
dc.description.urihttps://www.journals.ac.za/index.php/sajev/article/view/3420
dc.description.versionPublisher’s version
dc.format.extent9 pages
dc.identifier.citationSteyn, L. A. I., Addison, P. & Malan, A. P. 2019. Potential of South African entomopathogenic nematodes to control the leaf miner, Holocacista capensis (Lepidoptera: Heliozelidae). South African Journal of Enology and Viticulture, 40(2):301-309. doi:10.21548/40-2-3420
dc.identifier.issn2224-7904 (online)
dc.identifier.issn0253-939X (print)
dc.identifier.otherdoi:10.21548/40-2-3420
dc.identifier.urihttp://hdl.handle.net/10019.1/123440
dc.language.isoen_ZAen_ZA
dc.publisherMedPharm Publications
dc.rights.holderAuthors retain copyright
dc.subjectInsect pests -- Biological controlen_ZA
dc.subjectCocoonsen_ZA
dc.subjectEntomopathogenic nematodes -- Species -- South Africaen_ZA
dc.subjectHeterorhabditisen_ZA
dc.subjectIntegrated pest managementen_ZA
dc.subjectLeaf minersen_ZA
dc.subjectSteinernemaen_ZA
dc.titlePotential of South African entomopathogenic nematodes to control the leaf miner, Holocacista capensis (Lepidoptera: Heliozelidae)en_ZA
dc.typeArticleen_ZA
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