Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa
dc.contributor.author | Jacobs, Graeme Brendon | en_ZA |
dc.contributor.author | Bock, Stefanie | en_ZA |
dc.contributor.author | Schuch, Anita | en_ZA |
dc.contributor.author | Moschall, Rebecca | en_ZA |
dc.contributor.author | Schrom, Eva-Maria | en_ZA |
dc.contributor.author | Zahn, Juliane | en_ZA |
dc.contributor.author | Reuter, Christian | en_ZA |
dc.contributor.author | Preiser, Wolfgang | en_ZA |
dc.contributor.author | Rethwilm, Axel | en_ZA |
dc.contributor.author | Engelbrecht, Susan | en_ZA |
dc.contributor.author | Kerkau, Thomas | en_ZA |
dc.contributor.author | Bodem, Jochen | en_ZA |
dc.date.accessioned | 2013-07-03T08:18:52Z | |
dc.date.available | 2013-07-03T08:18:52Z | |
dc.date.issued | 2012-09-24 | |
dc.description | CITATION: Jacobs, G. B. et al. 2012. Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa. Viruses, 4(9):1830-1843; doi:10.3390/v4091830. | |
dc.description | The original publication is available at http://www.mdpi.com/journal/viruses | |
dc.description.abstract | The Human Immunodeficiency Virus type 1 (HIV-1) subtype C is currently the predominant subtype worldwide. Cell culture studies of Sub-Saharan African subtype C proviral plasmids are hampered by the low replication capacity of the resulting viruses, although viral loads in subtype C infected patients are as high as those from patients with subtype B. Here, we describe the sequencing and construction of a new HIV-1 subtype C proviral clone (pZAC), replicating more than one order of magnitude better than the previous subtype C plasmids. We identify the env-region for being the determinant for the higher viral titers and the pZAC Env to be M-tropic. This higher replication capacity does not lead to a higher cytotoxicity compared to previously described subtype C viruses. In addition, the pZAC Vpu is also shown to be able to down-regulate CD4, but fails to fully counteract CD317. | |
dc.description.uri | http://www.mdpi.com/1999-4915/4/9/1830 | |
dc.description.version | Publisher's version | |
dc.format.extent | 14 pages | |
dc.identifier.citation | Jacobs, G. B. et al. 2012. Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa. Viruses, 4(9):1830-1843; doi:10.3390/v4091830. | |
dc.identifier.issn | 1999-4915 (online) | |
dc.identifier.other | doi:10.3390/v4091830 | |
dc.identifier.uri | http://hdl.handle.net/10019.1/81483 | |
dc.language.iso | en_ZA | |
dc.publisher | MDPI | |
dc.rights.holder | Authors retain copyright | |
dc.subject | HIV-1 subtype C | en_ZA |
dc.subject | Proviral plasmid | en_ZA |
dc.subject | Viral replication | en_ZA |
dc.subject | Resistance assays | en_ZA |
dc.subject | Virusses -- Variations | en_ZA |
dc.subject | HIV (Viruses ) -- Research | en_ZA |
dc.title | Construction of a high titer infectious HIV-1 subtype C proviral clone from South Africa | en_ZA |
dc.type | Article |
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