Potential of host markers produced by infection phase-dependent antigen-stimulated cells for the diagnosis of tuberculosis in a highly endemic area

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dc.contributor.authorChegou, Novel N.en_ZA
dc.contributor.authorEssone, Paulin N.en_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorBlack, Gillian F.en_ZA
dc.contributor.authorVan der Spuy, Gian D.en_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorFranken, Kees L.en_ZA
dc.contributor.authorParida, Shreemanta K.en_ZA
dc.contributor.authorKlein, Michel R.en_ZA
dc.contributor.authorKaufmann, Stefan H. E.en_ZA
dc.contributor.authorOttenhoff, Tom H. M.en_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.date.accessioned2012-06-28T09:00:49Z
dc.date.available2012-06-28T09:00:49Z
dc.date.issued2012-06-05
dc.descriptionCITATION: Chegou, N. N. et al. 2012. Potential of host markers produced by infection phase-dependent antigen-stimulated cells for the diagnosis of tuberculosis in a highly endemic area. PLoS ONE, 7(6): e38501, doi:10.1371/journal.pone.0038501.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractBackground: Recent interferon gamma (IFN-γ)-based studies have identified novel Mycobacterium tuberculosis (M.tb) infection phase-dependent antigens as diagnostic candidates. In this study, the levels of 11 host markers other than IFN-γ, were evaluated in whole blood culture supernatants after stimulation with M.tb infection phase-dependent antigens, for the diagnosis of TB disease. Methodology and Principal Findings: Five M.tb infection phase-dependent antigens, comprising of three DosR-regulon-encoded proteins (Rv2032, Rv0081, Rv1737c), and two resucitation promoting factors (Rv0867c and Rv2389c), were evaluated in a case-control study with 15 pulmonary TB patients and 15 household contacts that were recruited from a high TB incidence setting in Cape Town, South Africa. After a 7-day whole blood culture, supernatants were harvested and the levels of the host markers evaluated using the Luminex platform. Multiple antigen-specific host markers were identified with promising diagnostic potential. Rv0081-specific levels of IL-12(p40), IP-10, IL-10 and TNF-α were the most promising diagnostic candidates, each ascertaining TB disease with an accuracy of 100%, 95% confidence interval for the area under the receiver operating characteristics plots, (1.0 to 1.0). Conclusions: Multiple cytokines other than IFN-γ in whole blood culture supernatants after stimulation with M.tb infection phase-dependent antigens show promise as diagnostic markers for active TB. These preliminary findings should be verified in well-designed diagnostic studies employing short-term culture assays. © 2012 Chegou et al.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0038501
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationChegou, N. N. et al. 2012. Potential of host markers produced by infection phase-dependent antigen-stimulated cells for the diagnosis of tuberculosis in a highly endemic area. PLoS ONE, 7(6): e38501, doi:10.1371/journal.pone.0038501.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0038501
dc.identifier.urihttp://hdl.handle.net/10019.1/21471
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosisen_ZA
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectCytokinesen_ZA
dc.titlePotential of host markers produced by infection phase-dependent antigen-stimulated cells for the diagnosis of tuberculosis in a highly endemic areaen_ZA
dc.typeArticle
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