Host markers in Quantiferon supernatants differentiate active TB from latent TB infection : preliminary report

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dc.contributor.authorChegou, Novel N.
dc.contributor.authorBlack, Gillian F.
dc.contributor.authorKidd, Martin
dc.contributor.authorVan Helden, Paul D.
dc.contributor.authorWalzl, Gerhard
dc.date.accessioned2010-12-13T08:37:11Z
dc.date.available2010-12-13T08:37:11Z
dc.date.issued2009-05
dc.date.updated2010-11-02T13:09:34Z
dc.descriptionIncludes bibliography
dc.description.abstractBackground: Interferon gamma release assays, including the QuantiFERON® TB Gold In Tube (QFT) have been shown to be accurate in diagnosing Mycobacterium tuberculosis infection. These assays however, do not discriminate between latent TB infection (LTBI) and active TB disease. Methods: We recruited twenty-three pulmonary TB patients and 34 household contacts from Cape Town, South Africa and performed the QFT test. To investigate the ability of new host markers to differentiate between LTBI and active TB, levels of 29 biomarkers in QFT supernatants were evaluated using a Luminex multiplex cytokine assay. Results: Eight out of 29 biomarkers distinguished active TB from LTBI in a pilot study. Baseline levels of epidermal growth factor (EGF) soluble CD40 ligand (sCD40L), antigen stimulated levels of EGF, and the background corrected antigen stimulated levels of EGF and macrophage inflammatory protein (MIP)-1β were the most informative single markers for differentiation between TB disease and LTBI, with AUCs of 0.88, 0.84, 0.87, 0.90 and 0.79 respectively. The combination of EGF and MIP-1β predicted 96% of active TB cases and 92% of LTBIs. Combinations between EGF, sCD40L, VEGF, TGF-α and IL-1α also showed potential to differentiate between TB infection states. EGF, VEGF, TGF-α and sCD40L levels were higher in TB patients. Conclusion: These preliminary data suggest that active TB may be accurately differentiated from LTBI utilizing adaptations of the commercial QFT test that includes measurement of EGF, sCD40L, MIP-1β, VEGF, TGF-α or IL-1α in supernatants from QFT assays. This approach holds promise for development as a rapid diagnostic test for active TB.en_ZA
dc.description.versionPeer Reviewed
dc.identifier.citationChegou, NN, Black, GF, Kidd, M, Van Helden, PD & Walzl, G 2009, 'Host markers in Quantiferon supernatants differentiate active TB from latent TB infection: preliminary report', BMC Pulmonary Medicine, 16;9(1):21.en_ZA
dc.identifier.issn1471-2466
dc.identifier.otherhttp://dx.doi.org/10.1186/1471-2466-9-21
dc.identifier.urihttp://hdl.handle.net/10019.1/5057
dc.language.isoen_USen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderChegou et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectInterferon gamma release assaysen_ZA
dc.subjectQuantiFERON® TB Gold In Tube (QFT)en_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subject.lcshBiochemical markersen_ZA
dc.titleHost markers in Quantiferon supernatants differentiate active TB from latent TB infection : preliminary reporten_ZA
dc.typeArticleen_ZA
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