Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism

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dc.contributor.authorMhelembe, Khethanien_ZA
dc.contributor.authorKriel, Johanen_ZA
dc.contributor.authorBester, Ceciliaen_ZA
dc.contributor.authorLouw, Esmeen_ZA
dc.contributor.authorRoodt-Wilding, Rouvayen_ZA
dc.contributor.authorTobutt, Kennethen_ZA
dc.date.accessioned2022-08-29T10:09:52Z
dc.date.available2022-08-29T10:09:52Z
dc.date.issued2020-02-07
dc.descriptionCITATION: Mhelembe K. et al. 2020. Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism. Horticultural Science (Prague), 47:69-79. doi:10.17221/83/2018-HORTSCI
dc.descriptionThe original publication is available at https://www.agriculturejournals.cz/web/hortsci/
dc.description.abstractThe ARC apple gene bank collection was genotyped for the fruit expressed gene ACS1, in which a short-interspersed element (SINE) in the promoter is known, when homozygous, to correlate with the delayed ethylene production. Primers were designed amplifying products less than 500 bp and 224 cultivars of domestic apple were analysed, 169 not previously genotyped. Of these, 82 were aa (homozygous for the high ethylene allele at 202 bp), 73 were ab and 14 bb (homozygous for the low ethylene allele, with the SINE, at 339 bp). The difference between the allele sizes, 137 bp, observed in the current study is consistent with the indel of 138 bp originally described, but differs considerably from the indel of 166 bp reported in literature. In addition, 21 accessions of other Malus species were analysed. Only one, M. ‘Golden Hornet’, had the b allele, which suggests it may have been introgressed from M. pumila.en_ZA
dc.description.urihttps://www.agriculturejournals.cz/web/hortsci.htm?type=article&id=83_2018-HORTSCI
dc.description.versionPublishers version
dc.format.extent11 pages
dc.identifier.citationMhelembe K. et al. 2020. Molecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphism. Horticultural Science (Prague), 47:69-79. doi:10.17221/83/2018-HORTSCI
dc.identifier.issn1805-9333 (online)
dc.identifier.issn0862-867X (print)
dc.identifier.otherdoi:10.17221/83/2018-HORTSCI
dc.identifier.urihttp://hdl.handle.net/10019.1/125663
dc.language.isoen_ZAen_ZA
dc.publisherCzech Academy of Agricultural Sciences
dc.subjectApplesen_ZA
dc.subjectFruit qualityen_ZA
dc.subjectGenotypingen_ZA
dc.subjectStorabilityen_ZA
dc.subjectMalus sp.en_ZA
dc.subjectMarker-assisted selectionen_ZA
dc.subjectApples -- Geneticsen_ZA
dc.subjectApples -- Storage -- Effect of gases onen_ZA
dc.titleMolecular characterisation of apple accessions with respect to aminocyclopropane-1-carboxylic acid synthase gene (ACS1) polymorphismen_ZA
dc.typeArticleen_ZA
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