G-protein regulation of the solubilized human zona pellucida-mediated acrosome reaction and zona pellucida binding

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dc.contributor.authorBastiaan H.
dc.contributor.authorFranken D.
dc.contributor.authorWranz P.
dc.date.accessioned2011-05-15T16:16:51Z
dc.date.available2011-05-15T16:16:51Z
dc.date.issued1999
dc.description.abstractPurpose: The study aimed to evaluate the (i) regulatory role of G(i)- like protein during the acrosome reaction (AR) of normal sperm donors and (ii) the role of intact acrosomes during sperm-zone binding. Methods: The acrosomal exocytosis of spermatozoa incubated with solubilized zone pellucida (ZP) at a final concentration of 1 ZP/μl was compared with 10 μM calcium ionophore A23187 and 30% (v/v) pooled human follicular fluid (HFF). Spermatozoa were incubated with 1, 10, and 100 ng/ml pertussis toxin (PT) during capacitation to functionally inactivate the G(i)-like protein. The sperm-zona binding potential of 100 ng/ml PT-treated spermatozoa followed, by exposure to 1 ZP/μl, revealed significantly higher zona-bound spermatozoa compared to controls treated with 1 ZP/μl only. Results: PT treatment of spermatozoa did not affect sperm motility, however, inhibited the percentage AR induced by solubilized ZP. In contrast, the A23187- and HFF-induced ARs were not sensitive to PT treatment. PT inhibition of the ZP-induced AR occurred in a concentration-dependent manner, with maximal effects observed at 100 ng/ml PT. Conclusions: In conclusion, it seems that PT-sensitive G(i)- like protein in human spermatozoa plays an important regulatory role in the AR induced by the human ZP, and this underlines the importance of intact acrosomes during sperm-zona binding.
dc.description.versionArticle
dc.identifier.citationJournal of Assisted Reproduction and Genetics
dc.identifier.citation16
dc.identifier.citation6
dc.identifier.issn10580468
dc.identifier.other10.1023/A:1020462201291
dc.identifier.urihttp://hdl.handle.net/10019.1/13963
dc.subjectcalcimycin
dc.subjectguanine nucleotide binding protein
dc.subjectpertussis toxin
dc.subjectacrosome reaction
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectexocytosis
dc.subjecthuman
dc.subjecthuman cell
dc.subjectnormal human
dc.subjectovary follicle fluid
dc.subjectpriority journal
dc.subjectregulatory mechanism
dc.subjectspermatozoon motility
dc.subjectzona pellucida
dc.subjectAcrosome Reaction
dc.subjectCalcimycin
dc.subjectExocytosis
dc.subjectFemale
dc.subjectFollicular Fluid
dc.subjectGTP-Binding Proteins
dc.subjectHumans
dc.subjectMale
dc.subjectOocytes
dc.subjectPertussis Toxin
dc.subjectSpermatozoa
dc.subjectVirulence Factors, Bordetella
dc.subjectZona Pellucida
dc.titleG-protein regulation of the solubilized human zona pellucida-mediated acrosome reaction and zona pellucida binding
dc.typeArticle
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