Inhibition of G protein in human sperm and its influence on acrosome reaction and zona pellucida binding

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dc.contributor.authorFranken D.R.
dc.contributor.authorMorales P.J.
dc.contributor.authorHabenicht U.F.
dc.date.accessioned2011-05-15T16:16:26Z
dc.date.available2011-05-15T16:16:26Z
dc.date.issued1996
dc.description.abstractObjective: To evaluate human sperm acrosomal status, zona pellucida (ZP)-binding capacity, and sperm motion characteristics after treatment with pertussis toxin followed by exposure to increasing concentrations of solubilized human ZP. Design: Prospective analytical study. Setting: Normal human sperm donors in an academic research environment. Intervention: Sperm were prepared with a wash and swim-up method and treated with a final concentration of 100 ng/mL pertussis toxin. Acrosomal status were determined using a Pisum sativum agglutinin-fluorescein-isothiocyanate method after exposure of sperm to 0.25, 0.5, 0.75, and 1.00 ZP/μL solutions of human ZP. Zona binding potential was recorded using intact zona-binding assays. Motion characteristics were recorded with a semen analyzer. Main Outcome Measure: Percentage acrosome-reacted sperm, number of zona-bound sperm, and sperm motion parameters. Results: Spermatozoa treated with 100 ng/mL pertussis toxin, followed by ZP-mediated acrosome reaction induction, showed a significant decrease in the percentage of acrosome-reacted sperm compared with untreated controls. Motion characteristics of 3-hour capacitated sperm after treatment with either phosphate-buffered saline (PBS) or pertussis toxin were not different. Pertussis toxin-treated sperm populations bound significantly more sperm to the ZP after 4 hours incubation compared with the PBS-control groups: 137.1 ± 8.0 compared with 96.3 ± 7.0 (mean ± SEM). Conclusions: The data support the concept of the controlling mechanism and importance of G proteins during the ZP-mediated acrosome reaction. Intact acrosomes correlate with and are needed to ensure tight zona binding.
dc.description.versionArticle
dc.identifier.citationFertility and Sterility
dc.identifier.citation66
dc.identifier.citation6
dc.identifier.issn00150282
dc.identifier.urihttp://hdl.handle.net/10019.1/13782
dc.subjectguanine nucleotide binding protein
dc.subjectpertussis toxin
dc.subjectacrosome
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectfemale
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectpriority journal
dc.subjectprotein determination
dc.subjectsemen analysis
dc.subjectspermatozoon capacitation
dc.subjectspermatozoon motility
dc.subjectspermatozoon penetration
dc.subjectzona pellucida
dc.subjectAcrosome
dc.subjectFemale
dc.subjectGTP-Binding Proteins
dc.subjectHumans
dc.subjectMale
dc.subjectPertussis Toxin
dc.subjectProspective Studies
dc.subjectSperm Motility
dc.subjectSperm-Ovum Interactions
dc.subjectSpermatozoa
dc.subjectVirulence Factors, Bordetella
dc.subjectZona Pellucida
dc.titleInhibition of G protein in human sperm and its influence on acrosome reaction and zona pellucida binding
dc.typeArticle
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