Successful TB treatment induces B-cells expressing FASL and IL5RA mRNA

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dc.contributor.authorVan Rensburg, Ilana C.en_ZA
dc.contributor.authorWagman, Chandreen_ZA
dc.contributor.authorStanley, Kimen_ZA
dc.contributor.authorBeltran, Carolineen_ZA
dc.contributor.authorRonacher, Katharinaen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorLoxton, Andre G.en_ZA
dc.date.accessioned2016-10-28T06:55:52Z
dc.date.available2016-10-28T06:55:52Z
dc.date.issued2016-09
dc.descriptionCITATION: Van Rensburg, I. C., et al. 2016. Successful TB treatment induces B-cells expressing FASL and IL5RA mRNA. Oncotarget, doi:10.18632/oncotarget.12184
dc.descriptionThe original publication is available at http://www.impactjournals.com/oncotarget
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.
dc.description.abstractENGLISH ABSTRACT: Activated B-cells increase T-cell behaviour during autoimmune disease and other infections by means of cytokine production and antigen-presentation. Functional studies in experimental autoimmune encephalomyelitis (EAE) indicate that B-cell deficiencies, and a lack of IL10 and IL35 leads to a poor prognosis. We hypothesised that B-cells play a role during tuberculosis. We evaluated B-cell mRNA expression using real-time PCR from healthy community controls, individuals with other lung diseases and newly diagnosed untreated pulmonary TB patients at three different time points (diagnosis, month 2 and 6 of treatment). We show that FASLG, IL5RA, CD38 and IL4 expression was lower in B-cells from TB cases compared to healthy controls. The changes in expression levels of CD38 may be due to a reduced activation of B-cells from TB cases at diagnosis. By month 2 of treatment, there was a significant increase in the expression of APRIL and IL5RA in TB cases. Furthermore, after 6 months of treatment, APRIL, FASLG, IL5RA and CD19 were upregulated in B-cells from TB cases. The increase in the expression of APRIL and CD19 suggests that there may be restored activation of B-cells following anti-TB treatment. The upregulation of FASLG and IL5RA indicates that B-cells expressing regulatory genes may play an important role in the protective immunity against M.tb infection. Our results show that increased activation of B-cells is present following successful TB treatment, and that the expression of FASLG and IL5RA could potentially be utilised as a signature to monitor treatment response.en_ZA
dc.description.urihttp://www.impactjournals.com/oncotarget/index.php?journal=oncotarget&page=article&op=view&path%5B%5D=12184&path%5B%5D=38572
dc.description.versionPublisher's version
dc.format.extent8 pages : illustrationsen_ZA
dc.identifier.citationVan Rensburg, I. C., et al. 2016. Successful TB treatment induces B-cells expressing FASL and IL5RA mRNA. Oncotarget, doi:10.18632/oncotarget.12184
dc.identifier.issn1949-2553 (online)
dc.identifier.otherdoi:10.18632/oncotarget.12184
dc.identifier.urihttp://hdl.handle.net/10019.1/99777
dc.language.isoen_ZAen_ZA
dc.publisherImpact Journalsen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectLungs -- Tuberculosis -- Treatmenten_ZA
dc.subjectMessenger RNAen_ZA
dc.subjectAutoimmune diseasesen_ZA
dc.subjectB cellsen_ZA
dc.titleSuccessful TB treatment induces B-cells expressing FASL and IL5RA mRNAen_ZA
dc.typeArticleen_ZA
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