Serum amyloid A binds to fibrin(ogen), promoting fibrin amyloid formation

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dc.contributor.authorPage, Martin J.en_ZA
dc.contributor.authorThomson, Greig J. A.en_ZA
dc.contributor.authorNunes, J. Massimoen_ZA
dc.contributor.authorEngelbrecht, Anna-Marten_ZA
dc.contributor.authorNell, Theo A.en_ZA
dc.contributor.authorDe Villiers, Willem J. S.en_ZA
dc.contributor.authorDe Beer, Maria C.en_ZA
dc.contributor.authorEngelbrecht, Lizeen_ZA
dc.contributor.authorKell, Douglas B.en_ZA
dc.contributor.authorPretorius, Etheresiaen_ZA
dc.date.accessioned2022-01-17T12:24:57Z
dc.date.available2022-01-17T12:24:57Z
dc.date.issued2019-02-28
dc.descriptionCITATION: Page, M. J., et al. 2019. Serum amyloid A binds to fibrin(ogen), promoting fibrin amyloid formation. Scientific Reports, 9:3102, doi:10.1038/s41598-019-39056-x.
dc.descriptionThe original publication is available at https://www.nature.com
dc.description.abstractComplex associations exist between inflammation and thrombosis, with the inflammatory state tending to promote coagulation. Fibrinogen, an acute phase protein, has been shown to interact with the amyloidogenic ß-amyloid protein of Alzheimer’s disease. However, little is known about the association between fibrinogen and serum amyloid A (SAA), a highly fibrillogenic protein that is one of the most dramatically changing acute phase reactants in the circulation. To study the role of SAA in coagulation and thrombosis, in vitro experiments were performed where purified human SAA, in concentrations resembling a modest acute phase response, was added to platelet-poor plasma (PPP) and whole blood (WB), as well as purified and fluorescently labelled fibrinogen. Results from thromboelastography (TEG) suggest that SAA causes atypical coagulation with a fibrin(ogen)-mediated increase in coagulation, but a decreased platelet/fibrin(ogen) interaction. In WB scanning electron microscopy analysis, SAA mediated red blood cell (RBC) agglutination, platelet activation and clumping, but not platelet spreading. Following clot formation in PPP, the presence of SAA increased amyloid formation of fibrin(ogen) as determined both with auto-fluorescence and with fluorogenic amyloid markers, under confocal microcopy. SAA also binds to fibrinogen, as determined with a fluorescent-labelled SAA antibody and correlative light electron microscopy (CLEM). The data presented here indicate that SAA can affect coagulation by inducing amyloid formation in fibrin(ogen), as well as by propelling platelets to a more prothrombotic state. The discovery of these multiple and complex effects of SAA on coagulation invite further mechanistic analyses.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-019-39056-x
dc.description.versionPublisher's version
dc.format.extent14 pages : illustrations (some color)en_ZA
dc.identifier.citationPage, M. J., et al. 2019. Serum amyloid A binds to fibrin(ogen), promoting fibrin amyloid formation. Scientific Reports, 9:3102, doi:10.1038/s41598-019-39056-x
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-019-39056-x
dc.identifier.urihttp://hdl.handle.net/10019.1/124093
dc.language.isoen_ZAen_ZA
dc.publisherNature Research (part of Springer Nature)
dc.rights.holderAuthors retain copyright
dc.subjectAmyloidogenicen_ZA
dc.subjectSerum amyloid Aen_ZA
dc.subjectFibrinogenen_ZA
dc.subjectAmyloid -- Analysisen_ZA
dc.titleSerum amyloid A binds to fibrin(ogen), promoting fibrin amyloid formationen_ZA
dc.typeArticleen_ZA
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