Characterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from Boza, and comparison of its efficiency to Pediocin PA-1

Simple item page
dc.contributor.authorMakhloufi, Kahina Maya
dc.contributor.authorCarre-Mlouka, Alyssa
dc.contributor.authorPeduzzi, Jean
dc.contributor.authorLombard, Carine
dc.contributor.authorVan Reenen, Carol Ann
dc.contributor.authorDicks, Leon Milner Theodore, 1961-
dc.contributor.authorRebuffat, Sylvie
dc.date.accessioned2013-10-22T06:24:52Z
dc.date.available2013-10-22T06:24:52Z
dc.date.issued2013-08
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund.en_ZA
dc.descriptionThe original publication is available at http://www.plosone.org/en_ZA
dc.description.abstractA bacteriocin-producing bacterium was isolated from boza and identified as Leuconostoc pseudomesenteroides KM432Bz. The antimicrobial peptide was purified and shown to be identical to other class IIa bacteriocins: leucocin A from Leuconostoc gelidum UAL-187 and Leuconostoc pseudomesenteroides QU15 and leucocin B from Leuconostoc carnosum Ta11a. The bacteriocin was named leucocin B-KM432Bz. Leucocin B-KM432Bz gene cluster encodes the bacteriocin precursor (lcnB), the immunity protein (lcnI) and the dedicated export machinery (lcnD and lcnE). A gene of unknown and non-essential function (lcnC), which is interrupted by an insertion sequence of the IS30 family, is localized between lcnB and lcnD. The activity of leucocin B-KM432Bz requires subunit C of the EIIt Man mannose permease, which is the receptor for entry into target cells. The determination of the minimum inhibitory concentrations revealed the lowest values for leucocin B-KM432Bz over Listeria strains, with 4 to 32 fold better efficiency than pediocin PA-1.en_ZA
dc.description.sponsorshipStellenbosch Universityen_ZA
dc.description.versionPublishers' versionen_ZA
dc.format.extent11 p. : ill.
dc.identifier.citationMakhloufi, K. M. et al. 2013. Characterization of leucocin B-KM432Bz from leuconostoc pseudomesenteroides isolated from boza, and comparison of its efficiency to pediocin PA-1. PloS ONE, 8(8):e70484, doi:10.1371/journal.pone.0070484.en_ZA
dc.identifier.issn1932-6203 (print)
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0070484
dc.identifier.urihttp://hdl.handle.net/10019.1/85465
dc.language.isoen_ZAen_ZA
dc.publisherPLoSen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectBoza (fermented beverage) -- Microbiologyen_ZA
dc.subjectLeucocin B-KM432Bz (bacteriocin)en_ZA
dc.subjectAntimicrobial peptidesen_ZA
dc.subjectLactic acid bacteriaen_ZA
dc.titleCharacterization of Leucocin B-KM432Bz from Leuconostoc pseudomesenteroides isolated from Boza, and comparison of its efficiency to Pediocin PA-1en_ZA
dc.typeArticleen_ZA
Files
Collections
Research Articles (Microbiology)