Mycobacterial transport medium for routine culture of fine needle aspiration biopsies

dc.contributor.authorWright, C. A.
dc.contributor.authorBamford, C.
dc.contributor.authorPrince, Y.
dc.contributor.authorVermaak, A.
dc.contributor.authorHoek, K. G. P.
dc.contributor.authorMarais, B. J.
dc.contributor.authorWarren, Roben_ZA
dc.descriptionThe original publication is available at
dc.description.abstractFine needle aspiration biopsy (FNAB) offers a simple outpatient technique for specimen collection in child tuberculosis suspects with peripheral lymphadenopathy. To perform FNAB with mycobacterial culture on an outpatient basis requires use of a sterile transport medium to facilitate bedside inoculation, maintain organism viability and reduce contamination risk en route to the laboratory. The mycobacterial yield and time to positive culture following bedside inoculation into standard mycobacterial growth indicator tubes were compared with initial inoculation into an inexpensive "in-house" liquid growth medium. Of 150 FNAB performed, 57 (38%) cultured Mycobacterium tuberculosis complex. There was one case each with non-tuberculous mycobacteria and Mycobacterium bovis BCG; the remaining 55 being M tuberculosis. Results were concordant in 142 (94.7%) bedside and laboratory inoculation pairs. There was no significant difference in time to positive culture between bedside and laboratory inoculation (16.2 days (SD 0.87) vs 17.1 days (SD 0.85)). Provision of inexpensive specimen transport bottles and practical tuition in FNAB should improve cost-effective diagnosis of tuberculosis at the primary healthcare level.en_ZA
dc.description.sponsorshipNational Health Laboratory Services, South Africaen_ZA
dc.format.extent6 p. : ill.
dc.identifier.citationWright, C. a. et al. 2010. Mycobacterial transport medium for routine culture of fine needle aspiration biopsies. Archives of Disease in Childhood, 95(1):48-50, doi:10.1136/adc.2009.164038.en_ZA
dc.identifier.issn1468-2044 (online)
dc.publisherBMJ Publishing Group
dc.rights.holderBMJ Publishing Group
dc.subjectMycobacterium tuberculosis in childrenen_ZA
dc.subjectFine needle aspiration biopsy (FNAB)en_ZA
dc.subjectChild (Preschool)en_ZA
dc.subjectLymph node tuberculosisen_ZA
dc.subjectPeripheral lymphadenopathy -- Diagnosisen_ZA
dc.subjectTuberculosis in children -- Diagnosisen_ZA
dc.subjectMycobacterial culture -- Identificationen_ZA
dc.titleMycobacterial transport medium for routine culture of fine needle aspiration biopsiesen_ZA
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