Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana

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dc.contributor.authorMogashoa, Tueloen_ZA
dc.contributor.authorMelamu, Pinkieen_ZA
dc.contributor.authorLey, Serej D.en_ZA
dc.contributor.authorStreicher, Elizabeth M.en_ZA
dc.contributor.authorIketleng, Thatoen_ZA
dc.contributor.authorKelentse, Nametsoen_ZA
dc.contributor.authorMupfumi, Lucyen_ZA
dc.contributor.authorMokomane, Margareten_ZA
dc.contributor.authorKgwaadira, Botsheloen_ZA
dc.contributor.authorNovitsky, Vladimiren_ZA
dc.contributor.authorKasvosve, Ishmaelen_ZA
dc.contributor.authorMoyo, Sikhulileen_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorGaseitsiwe, Simanien_ZA
dc.date.accessioned2021-06-29T08:02:04Z
dc.date.available2021-06-29T08:02:04Z
dc.date.issued2019-05-07
dc.descriptionCITATION: Mogashoa, T. et al. 2019. Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana. PLoS ONE, 14(5):e0216306, doi:10.1371/journal.pone.0216306.
dc.descriptionThe original publication is available at https://journals.plos.org/plosone
dc.description.abstractBackground: Molecular typing of Mycobacterium tuberculosis (M.tb) isolates can inform Tuberculosis (TB) control programs on the relative proportion of transmission driving the TB epidemic. There is limited data on the M. tb genotypes that are circulating in Botswana. The aim of this study was to generate baseline data on the genetic diversity of M.tb isolates circulating in the country. Methods: A total of 461 M.tb isolates received at the Botswana National Tuberculosis Reference Laboratory between March 2012 and October 2013 were included in this study. Drug susceptibility testing was conducted using the BD BACTEC MGIT 960 System. M.tb strains were genotyped using spoligotyping and spoligotype patterns were compared with existing patterns in the SITVIT Web database. A subset of drug resistant isolates which formed spoligo clusters (n = 65) was additionally genotyped with 12-loci MIRU. Factors associated with drug resistance and clustering were evaluated using logistic regression. Results: Of the 461 isolates genotyped, 458 showed 108 distinct spoligotype patterns. The predominant M.tb lineages were Lineage 4 (81.9%), Lineage 2 (9%) and Lineage 1 (7.2%). The predominant spoligotype families within Lineage 4 were LAM (33%), S (14%), T (16%), X (16%). Three hundred and ninety-two (86%) isolates could be grouped into 44 clusters (2– 46 isolates per cluster); giving a clustering rate of 76%. We identified 173 (37.8%) drug resistant isolates, 48 (10.5%) of these were multi-drug resistant. MIRU typing of the drug resistant isolates allowed grouping of 46 isolates into 14 clusters, giving a clustering rate of 49.2%. There was no association between age, sex, treatment category, region and clustering. Conclusions: This study highlights the complexity of the TB epidemic in Botswana with multiple strains contributing to disease and provides baseline data on the population structure of M.tb strains in Botswana.en_ZA
dc.description.sponsorshipSub-Saharan African Network for TB/HIV Research Excellence (SANTHE)
dc.description.sponsorshipDELTAS Africa
dc.description.versionPublisher's version
dc.format.extent14 pagesen_ZA
dc.identifier.citationMogashoa, T. et al. 2019. Genetic diversity of Mycobacterium tuberculosis strains circulating in Botswana. PLoS ONE, 14(5):e0216306, doi:10.1371/journal.pone.0216306.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0216306
dc.identifier.urihttp://hdl.handle.net/10019.1/110624
dc.language.isoen_ZAen_ZA
dc.publisherPLoSen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectGenetic diseasesen_ZA
dc.subjectEpidemic -- TB diseasesen_ZA
dc.subjectMolecular virologyen_ZA
dc.titleGenetic diversity of Mycobacterium tuberculosis strains circulating in Botswanaen_ZA
dc.typeArticleen_ZA
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