The development of one- and two-cell mouse embryos in the absence of human serum

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dc.contributor.authorKruger, T. F.en_ZA
dc.contributor.authorStander, F. S. H.en_ZA
dc.contributor.authorSmith, K.en_ZA
dc.contributor.authorLombard, C. J.en_ZA
dc.date.accessioned2011-03-18T14:57:30Z
dc.date.available2011-03-18T14:57:30Z
dc.date.issued1986
dc.descriptionCITATION: Kruger, T. F. et al. 1986. The development of one- and two-cell mouse embryos in the absence of human serum. South African Medical Journal, 70:542-543.
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractOne- and two-cell embryos were obtained from F1 hybrid female mice stimulated with human menopausal gonadotrophin and randomly distributed into two groups - group 1 (no serum) and group 2 (10% patient's serum). Fifty of 53 (94.3%) one-cell embryos in group 1 had cleaved to the blastocyst stage and 44 of 49 (89.79%) in group 2 after 96 hours (no significant difference - chi-square test). In the two-cell embryos in group 1, 78 of 89 (87.6%) reached the blastocyst stage after 72 hours and 80 of 86 (93.02%) in group 2 (no significant difference - chi-square test). According to microscopic evaluation cleavage to the blastocyst stage without serum supplementation is possible. The value of serum is discussed.
dc.description.versionPublisher’s version
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7461
dc.language.isoen
dc.publisherHealth & Medical Publishing Group
dc.rights.holderSouth African Medical Journal
dc.subjectEmbryosen_ZA
dc.titleThe development of one- and two-cell mouse embryos in the absence of human serumen_ZA
dc.typeArticle
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