Binding of deoxy-thymidine-5'-triphosphate and deoxyadenosine-5'-triphosphate to chrysotile asbestos

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dc.contributor.authorHawtrey, A.en_ZA
dc.contributor.authorPieterse, A.en_ZA
dc.contributor.authorVan Zyl, J. M.en_ZA
dc.contributor.authorBester, A. J.en_ZA
dc.contributor.authorHarington, J. S.en_ZA
dc.date.accessioned2013-01-23T13:46:26Zen_ZA
dc.date.available2013-01-23T13:46:26Zen_ZA
dc.date.issued2003-12en_ZA
dc.descriptionCITATION: Hawtrey, A. et al. 2002. Binding of deoxy-thymidine-5'-triphosphate and deoxyadenosine-5'-triphosphate to chrysotile asbestos. South African Journal of Science, 98(11).en_ZA
dc.description.abstractABSTRACT: Tritiated deoxythymidine-5'-triphosphate ([3H]dTTP) and deoxyadenosine- 5'-triphosphate ([3H]dATP) were found to bind strongly to fibres of chrysotile asbestos. Binding of both triphosphates to amosite and crocidolite, however, was much less than that to chrysotile. Further work with chrysotile showed that the binding appears to be electrostatic in nature, with the negative phosphate groups of the nucleotides attaching to the positively charged groups on the mineral fibre. Attachment of the triphosphates to the mineral surface was found to be concentration dependent. Time curves of binding indicated that, for both nucleoside triphosphates, binding was rapid at 20oC and 37oC. Strong binding was also observed at 55oC. Binding at the higher temperatures depended on the type of buffer used and pH. Bound [3H]dTTP and [3H]dATP could be partially removed from chrysotile by 0.1 M sodium pyrophosphate after a short incubation at 20oC. Various chemical reagents that affected binding were studied and are discussed. The possible use of chrysotile asbestos as a solid phase material for the polymerization of dTTP and dATP or activated 5'-phosphorimidazolide of adenosine is also discussed.en_ZA
dc.description.versionPublisher's versionen_ZA
dc.format.extent3 pages : illustrationsen_ZA
dc.identifier.citationHawtrey, A. et al. 2002. Binding of deoxy-thymidine-5'-triphosphate and deoxyadenosine-5'-triphosphate to chrysotile asbestos. South African Journal of Science, 98(11).en_ZA
dc.identifier.issn1996-7489 (online)en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/76492en_ZA
dc.language.isoenen_ZA
dc.publisherAcademy of Science for South Africaen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subject.lcshPolymerizationen_ZA
dc.subject.lcshMontmorillonite catalystsen_ZA
dc.subject.lcshChrysotileen_ZA
dc.subject.lcshAsbestosen_ZA
dc.subject.lcshUCTDen_ZA
dc.titleBinding of deoxy-thymidine-5'-triphosphate and deoxyadenosine-5'-triphosphate to chrysotile asbestosen_ZA
dc.typeArticleen_ZA
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