Diverse exopolysaccharide producing bacteria isolated from milled sugarcane : implications for cane spoilage and sucrose yield

Simple item page
dc.contributor.authorHector, Stantonen_ZA
dc.contributor.authorWillard, Kyleen_ZA
dc.contributor.authorBauer, Roleneen_ZA
dc.contributor.authorMulako, Inongeen_ZA
dc.contributor.authorSlabbert, Etienneen_ZA
dc.contributor.authorKossmann, Jensen_ZA
dc.contributor.authorGeorge, Gavin M.en_ZA
dc.date.accessioned2016-08-12T09:03:07Z
dc.date.available2016-08-12T09:03:07Z
dc.date.issued2015
dc.descriptionCITATION: Hector, S., et al. 2015. Diverse exopolysaccharide producing bacteria isolated from milled sugarcane : implications for cane spoilage and sucrose yield. PLoS ONE, 10(12):1-10, doi:10.1371/journal.pone.0145487.
dc.descriptionThe original publication is available at http://journals.plos.org/plosone
dc.description.abstractBacterial deterioration of sugarcane during harvesting and processing is correlated with significant loss of sucrose yield and the accumulation of bacterial polysaccharides. Dextran, a homoglucan produced by Leuconostoc mesenteroides, has been cited as the primary polysaccharide associated with sugarcane deterioration. A culture-based approach was used to isolate extracellular polysaccharide (EPS) producing bacterial strains from milled sugarcane stalks. Ribosomal RNA sequencing analysis grouped 25 isolates into 4 genera. This study identified 2 bacterial genera not previously associated with EPS production or sucrose degradation. All isolates produced polysaccharide when grown in the presence of sucrose. Monosaccharide analysis of purified polymers by Gas Chromatography revealed 17 EPSs consisting solely of glucose (homoglucans), while the remainder contained traces of mannose or fructose. Dextranase treatment of polysaccharides yielded full digestion profiles for only 11 extracts. Incomplete hydrolysis profiles of the remaining polysaccharides suggest the release of longer oligosaccharides which may interfere with sucrose crystal formation.en_ZA
dc.description.sponsorshipSouth African National Research Foundation—Innovation Fund (http://www.nrf.ac.za/)en_ZA
dc.description.sponsorshipZurich-Basel Plant Science Centers’ Plant Fellows program (Grant agreement no GA-2010-267243)en_ZA
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0145487
dc.description.versionPublisher's version
dc.format.extent10 pages
dc.identifier.citationHector, S., et al. 2015. Diverse exopolysaccharide producing bacteria isolated from milled sugarcane : implications for cane spoilage and sucrose yield. PLoS ONE, 10(12):1-10, doi:10.1371/journal.pone.0145487
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0145487
dc.identifier.urihttp://hdl.handle.net/10019.1/99363
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectBacterial deteriorationen_ZA
dc.subjectSugarcaneen_ZA
dc.subjectExopolysaccharideen_ZA
dc.subjectSucroseen_ZA
dc.titleDiverse exopolysaccharide producing bacteria isolated from milled sugarcane : implications for cane spoilage and sucrose yielden_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
hector_diverse_2015.pdf
Size:
2.65 MB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.95 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Genetics)