The specific binding of the thyroid hormones to matrix isolated from rat liver nuclei

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dc.contributor.authorWilson, B. D.en_ZA
dc.contributor.authorAlbrecht, C. F.en_ZA
dc.contributor.authorWium, C. A.en_ZA
dc.date.accessioned2011-03-18T14:57:45Z
dc.date.available2011-03-18T14:57:45Z
dc.date.issued1982
dc.descriptionCITATION: Wilson, B. D., Albrecht, C. F. & Wium, C. A. 1982. The specific binding of the thyroid hormones to matrix isolated from rat liver nuclei. South African Medical Journal, 61:44-49.
dc.descriptionThe original publication is available at http://www.samj.org.za
dc.description.abstractSpecific binding sites for the thyroid hormones have been demonstrated in the liver nuclear matrix, a structural framework of the nucleus. When labelled 3,5,3'-tri-iodo-L-thyronine ([125I]T3) is injected into rats, 5% of the total nucleus bound T3 is bound to the matrix after 1 hour. However, when either isolated nuclei or isolated nuclear matrices were incubated with [125I]T3 in vitro, a 3- to 7-fold greater number of specific T3 binding sites were revealed in the nuclear matrix. The proporties of the matrix-associated thyroid hormone binding sites were investigated in vitro. These binding sites showed limited capacity and high affinity for T3; the equilibrium association constant (K(a)) was 1.3 x 109 M-1 and the binding capacity was 20.2 fmol T3 per 100 μg matrix protein.
dc.description.versionPublisher’s version
dc.format.extent6 pages
dc.identifier.issn2078-5135 (online)
dc.identifier.issn0256-9574 (print)
dc.identifier.urihttp://hdl.handle.net/10019.1/7624
dc.language.isoen
dc.publisherHealth & Medical Publishing Group
dc.rights.holderSouth African Medical Journal
dc.subjectThyroid hormonesen_ZA
dc.titleThe specific binding of the thyroid hormones to matrix isolated from rat liver nucleien_ZA
dc.typeArticle
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