Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa

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dc.contributor.authorMatsha, Tandi E.en_ZA
dc.contributor.authorPheiffer, Carmenen_ZA
dc.contributor.authorHumphries, Stephen E.en_ZA
dc.contributor.authorGamieldien, Junaiden_ZA
dc.contributor.authorErasmus, Rajiv T.en_ZA
dc.contributor.authorKengne, Andre P.en_ZA
dc.date.accessioned2017-08-24T13:10:13Z
dc.date.available2017-08-24T13:10:13Z
dc.date.issued2016
dc.descriptionCITATION: Matsha, T. E., et al. 2016. Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa. International Journal of Endocrinology, 2016 (Article ID 3172093), doi:10.1155/2016/3172093.en_ZA
dc.descriptionThe original publication is available at https://www.hindawi.comen_ZA
dc.description.abstractAims. To conduct a genome-wide DNA methylation in individuals with type 2 diabetes, individuals with prediabetes, and control mixed ancestry individuals from South Africa. Methods. We used peripheral blood to perform genome-wide DNA methylation analysis in 3 individuals with screen detected diabetes, 3 individuals with prediabetes, and 3 individuals with normoglycaemia from the Bellville South Community, Cape Town, South Africa, who were age-, gender-, body mass index-, and duration of residency-matched. Methylated DNA immunoprecipitation (MeDIP) was performed by Arraystar Inc. (Rockville, MD, USA). Results. Hypermethylated DMRs were 1160 (81.97%) and 124 (43.20%), respectively, in individuals with diabetes and prediabetes when both were compared to subjects with normoglycaemia. Our data shows that genes related to the immune system, signal transduction, glucose transport, and pancreas development have altered DNA methylation in subjects with prediabetes and diabetes. Pathway analysis based on the functional analysis mapping of genes to KEGG pathways suggested that the linoleic acid metabolism and arachidonic acid metabolism pathways are hypomethylated in prediabetes and diabetes. Conclusions. Our study suggests that epigenetic changes are likely to be an early process that occurs before the onset of overt diabetes. Detailed analysis of DMRs that shows gradual methylation differences from control versus prediabetes to prediabetes versus diabetes in a larger sample size is required to confirm these findings.en_ZA
dc.description.urihttps://www.hindawi.com/journals/ije/2016/3172093/
dc.description.versionPublisher's versionen_ZA
dc.format.extent11 pages : illustrations (some colour)en_ZA
dc.identifier.citationMatsha, T. E., et al. 2016. Genome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africa. International Journal of Endocrinology, 2016 (Article ID 3172093), doi:10.1155/2016/3172093en_ZA
dc.identifier.issn1687-8345 (online)
dc.identifier.issn1687-8337 (print)
dc.identifier.otherdoi:10.1155/2016/3172093
dc.identifier.urihttp://hdl.handle.net/10019.1/102143
dc.language.isoen_ZAen_ZA
dc.publisherHindawi Publishing Corporationen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectDiabetes -- South Africa -- Western Capeen_ZA
dc.subjectGenome-wide DNA methylation analysisen_ZA
dc.subjectNon-insulin-dependent diabetes -- South Africa -- Western Capeen_ZA
dc.titleGenome-wide DNA methylation in mixed ancestry individuals with diabetes and prediabetes from South Africaen_ZA
dc.typeArticleen_ZA
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