Rapid, field-deployable method for collecting and preserving plant metabolome for biochemical and functional characterization

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dc.contributor.authorSkubel, Sarah A.en_ZA
dc.contributor.authorDushenkov, Vyacheslaven_ZA
dc.contributor.authorGraf, Brittany L.en_ZA
dc.contributor.authorNiu, Qingweien_ZA
dc.contributor.authorPoulev, Alexanderen_ZA
dc.contributor.authorKalariya, Hetalben M.en_ZA
dc.contributor.authorFoxcroft, Llewellyn C.en_ZA
dc.contributor.authorRaskin, Ilyaen_ZA
dc.date.accessioned2019-10-04T11:52:29Z
dc.date.available2019-10-04T11:52:29Z
dc.date.issued2018-09-06
dc.descriptionCITATION: Skubel, S. A. et al. 2018. Rapid, field-deployable method for collecting and preserving plant metabolome for biochemical and functional characterization. PLoS ONE, 13(9):e0203569, doi:10.1371/journal.pone.0203569.en_ZA
dc.descriptionThe original publication is available at https://journals.plos.orgen_ZA
dc.description.abstractStudy of plant metabolome is a growing field of science that catalogs vast biochemical and functional diversity of phytochemicals. However, collecting and storing samples of plant metabolome, sharing these samples across the scientific community and making them compatible with bioactivity assays presents significant challenges to the advancement of metabolome research. We have developed a RApid Metabolome Extraction and Storage (RAMES) technology that allows efficient, highly compact, field-deployable collection and storage of libraries of plant metabolome. RAMES technology combines rapid extraction with immobilization of extracts on glass microfiber filter discs. Two grams of plant tissue extracted in ethanol, using a specially adapted Dremel® rotary tool, produces 25–35 replicas of 10 mm glass fiber discs impregnated with phytochemicals. These discs can be either eluted with solvents (such as 70% ethanol) to study the metabolomic profiles or used directly in a variety of functional assays. We have developed simple, non-sterile, anti-fungal, anti-bacterial, and anti-oxidant assays formatted for 24-multiwell plates directly compatible with RAMES discs placed inside the wells. Using these methods we confirmed activity in 30 out of 32 randomly selected anti-microbial medicinal plants and spices. Seven species scored the highest activity (total kill) in the anti-bacterial (bacteria from human saliva) and two anti-fungal screens (Fusarium spp. and Saccharomyces cerevisiae), providing functional validation of RAMES technology. RAMES libraries showed limited degradation of compounds after 12 months of storage at -20°C, while others remained stable. Fifty-eight percent of structures characterized in the extracts loaded onto RAMES discs could be eluted from the discs without significant losses. Miniaturized RAMES technology, as described and validated in this manuscript offers a labor, cost, and time-effective alternative to conventional collection of phytochemicals. RAMES technology enables creation of comprehensive metabolomic libraries from various ecosystems and geographical regions in a format compatible with further biochemical and functional studies.en_ZA
dc.description.sponsorshipT.R.E.E. International Institute, Miami, FL.en_ZA
dc.description.sponsorshipHostos Community College, City University of New Yorken_ZA
dc.description.sponsorshipSouth African National Parks, the DST-NRF Centre of Excellence for Invasion Biology (C·I·B)en_ZA
dc.description.sponsorshipNational Research Foundation of South Africaen_ZA
dc.description.urihttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0203569
dc.description.versionPublisher's versionen_ZA
dc.format.extent19 pages : illustrationsen_ZA
dc.identifier.citationSkubel, S. A. et al. 2018. Rapid, field-deployable method for collecting and preserving plant metabolome for biochemical and functional characterization. PLoS ONE, 13(9):e0203569, doi:10.1371/journal.pone.0203569.en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0203569
dc.identifier.urihttp://hdl.handle.net/10019.1/106578
dc.language.isoen_ZAen_ZA
dc.publisherPublic Library of Scienceen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectPlant metabolomeen_ZA
dc.subjectPhytochemicalsen_ZA
dc.subjectRapid Metabolome Extraction and Storage (RAMES) technologyen_ZA
dc.titleRapid, field-deployable method for collecting and preserving plant metabolome for biochemical and functional characterizationen_ZA
dc.typeArticleen_ZA
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