Tanshinone production could be increased by the expression of SmWRKY2 in Salvia miltiorrhiza hairy roots

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dc.contributor.authorDeng, Changpingen_ZA
dc.contributor.authorHao, Xiaolongen_ZA
dc.contributor.authorShi, Minen_ZA
dc.contributor.authorFu, Rongen_ZA
dc.contributor.authorWang, Yaoen_ZA
dc.contributor.authorZhang, Yien_ZA
dc.contributor.authorZhou, Weien_ZA
dc.contributor.authorFeng, Yueen_ZA
dc.contributor.authorMakunga, Nokwanda P.en_ZA
dc.contributor.authorKai, Guoyinen_ZA
dc.date.accessioned2021-10-26T11:57:53Z
dc.date.available2021-10-26T11:57:53Z
dc.date.issued2019
dc.descriptionCITATION: Deng, C. et al. 2019. Tanshinone production could be increased by the expression of SmWRKY2 in Salvia miltiorrhiza hairy roots. Plant Science, 284:1-8. doi:10.1016/j.plantsci.2019.03.007
dc.descriptionThe original publication is available at https://www.sciencedirect.com/journal/plant-science
dc.description.abstractTanshinones are the main bioactive diterpenes in Salvia miltiorrhiza Bunge, are widely used for treating cardiovascular and cerebrovascular diseases. However, the biosynthetic mechanisms of these compounds have not yet been fully explained. In this study, a transcription factor named SmWRKY2 was isolated and functionally characterized. Multiple sequence analysis indicated it was classified into subgroup I of the WRKY family. Expression pattern showed that SmWRKY2 was mainly expressed in the stem and leaf and was inducible by methyl jasmonate (MeJA) treatment. Subcellular localization showed that SmWRKY2 was localized in the nucleus. Overexpression of SmWRKY2 in S. miltiorrhiza hairy roots significantly increased the expression of SmDXS2 and SmCPS, resulting in increased accumulation of tanshinones and the highest total tanshinone content was detected in OE-SmWRKY2-1 line, which was 1.83 times of the control. Meanwhile, tanshinone production was slightly reduced in the antisense-SmWRKY2 line. Dual-Luciferase assay showed that SmWRKY2 can positively regulate SmDXS2 and SmCPS expression, However, Y1H and EMSA experiments indicate that SmWRKY2 only binds to the W-box of the SmCPS promoter. Our study shows that SmWRKY2 is a positive regulator of tanshinone biosynthesis by mainly activating SmCPS. This study thus sheds new light on the regulatory role of SmWRKY2 in tanshinone biosynthesis.en_ZA
dc.description.urihttps://www.sciencedirect.com/science/article/pii/S0168945218314961?via%3Dihub
dc.description.versionPublisher's version
dc.format.extent8 pages : illustrations (some color)en_ZA
dc.identifier.citationDeng, C. et al. 2019. Tanshinone production could be increased by the expression of SmWRKY2 in Salvia miltiorrhiza hairy roots. Plant Science, 284:1-8. doi:10.1016/j.plantsci.2019.03.007
dc.identifier.issn0168-9452 (print)
dc.identifier.otherdoi:10.1016/j.plantsci.2019.03.007
dc.identifier.urihttp://hdl.handle.net/10019.1/123313
dc.language.isoen_ZAen_ZA
dc.publisherElsevier
dc.rights.holderAuthors retain rights
dc.subjectHairy rootsen_ZA
dc.subjectS.miltiorrhizaen_ZA
dc.subjectTanshinonesen_ZA
dc.subjectWRKY transcription factoren_ZA
dc.subjectHairy-root diseaseen_ZA
dc.subjectCardiovascular disease -- Treatmenten_ZA
dc.titleTanshinone production could be increased by the expression of SmWRKY2 in Salvia miltiorrhiza hairy rootsen_ZA
dc.typeArticleen_ZA
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