Cell-autonomous role of GFRα1 in the development of olfactory bulb GABAergic interneurons

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dc.contributor.authorZechel, Sabrinaen_ZA
dc.contributor.authorFernandez-Suarez, Dianaen_ZA
dc.contributor.authorIbanez, Carlos F.en_ZA
dc.date.accessioned2019-09-02T06:38:42Z
dc.date.available2019-09-02T06:38:42Z
dc.date.issued2018-05-18
dc.descriptionCITATION: Zechel, S., Fernandez-Suarez, D. & Ibanez, C. F. 2018. Cell-autonomous role of GFRα1 in the development of olfactory bulb GABAergic interneurons. Biology Open, 7:bio033753, doi:10.1242/bio.033753.
dc.descriptionThe original publication is available at https://bio.biologists.org
dc.description.abstractGFRα1, a receptor for glial cell line-derived neurotrophic factor (GDNF), is critical for the development of the main olfactory system. The olfactory bulb (OB) of Gfra1 knockout mice shows significant reductions in the number of olfactory sensory neurons, mitral and tufted cells, as well as all major classes of OB GABAergic interneurons. However, the latter do not express significant levels of GFRα1, leaving the mechanism of action of GFRα1 in OB interneuron development unexplained. Here we report that GFRα1 is highly expressed in the precursor cells that give rise to all major classes of OB interneurons, but is downregulated as these neurons mature. Conditional ablation of GFRα1 in embryonic GABAergic cells recapitulated the cell losses observed in global Gfra1 knockouts at birth. GFRα1 was also required for the sustained generation and allocation of OB interneurons in adulthood. Conditional loss of GFRα1 altered the migratory behaviour of neuroblasts along the rostral migratory stream (RMS) as well as RMS glial tunnel formation. Together, these data indicate that GFRα1 functions cellautonomously in subpopulations of OB interneuron precursors to regulate their generation and allocation in the mammalian OB.en_ZA
dc.description.sponsorshipSwedish Research Council
dc.description.sponsorshipSwedish Cancer Society
dc.description.sponsorshipKnut and Alice Wallenbergs Foundation
dc.description.sponsorshipStrategic Research Program in Regenerative Medicine KI
dc.description.sponsorshipNational University of Singapore
dc.description.sponsorshipWenner-Gren Foundation
dc.description.urihttps://bio.biologists.org/content/7/5/bio033753
dc.description.versionPublisher's version
dc.format.extent11 pages
dc.identifier.citationZechel, S., Fernandez-Suarez, D. & Ibanez, C. F. 2018. Cell-autonomous role of GFRα1 in the development of olfactory bulb GABAergic interneurons. Biology Open, 7:bio033753, doi:10.1242/bio.033753.
dc.identifier.issn2046-6390 (online)
dc.identifier.otherdoi:10.1242/bio.033753
dc.identifier.urihttp://hdl.handle.net/10019.1/106409
dc.language.isoen_ZAen_ZA
dc.publisherThe Company of Biologists
dc.rights.holderAuthors retain copyright
dc.subjectCell migrationen_ZA
dc.subjectRostral migratory streamen_ZA
dc.subjectSubventricular zoneen_ZA
dc.subjectInterneuronsen_ZA
dc.subjectOlfactory interneuronsen_ZA
dc.subjectOlfactory bulben_ZA
dc.subjectGABA -- Receptorsen_ZA
dc.titleCell-autonomous role of GFRα1 in the development of olfactory bulb GABAergic interneuronsen_ZA
dc.typeArticleen_ZA
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