Species interaction and selective carbon addition during antibiotic exposure enhances bacterial survival

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dc.contributor.authorJackson, Lindsay M. D.en_ZA
dc.contributor.authorKroukamp, Otinien_ZA
dc.contributor.authorYeung, William C.en_ZA
dc.contributor.authorRonan, Evanen_ZA
dc.contributor.authorLiss, Steven N.en_ZA
dc.contributor.authorWolfaardt, Gideon M.en_ZA
dc.date.accessioned2021-11-03T12:57:46Z
dc.date.available2021-11-03T12:57:46Z
dc.date.issued2019-11-29
dc.descriptionCITATION: Jackson, L. D. M., et al. 2021. Species interaction and selective carbon addition during antibiotic exposure enhances bacterial survival. Frontiers in Microbiology, 10:2730, doi:10.3389/fmicb.2019.02730.
dc.descriptionThe original publication is available at https://www.frontiersin.org
dc.description.abstractBiofilms are multifaceted and robust microbiological systems that enable microorganisms to withstand a multitude of environmental stresses and expand their habitat range. We have shown previously that nutritional status alters antibiotic susceptibility in a mixed-species biofilm. To further elucidate the effects of nutrient addition on inter-species dynamics and whole-biofilm susceptibility to high-dose streptomycin exposures, a CO2 Evolution Measurement System was used to monitor the metabolic activity of early steady state pure-culture and mixed-species biofilms containing Pseudomonas aeruginosa and Stenotrophomonas maltophilia, with and without added carbon. Carbon supplementation was needed for biofilm recovery from high-dose streptomycin exposures when P. aeruginosa was either the dominant community member in a mixed-species biofilm (containing predominantly P. aeruginosa and S. maltophilia) or as a pure culture. By contrast, S. maltophilia biofilms could recover from high-dose streptomycin exposures without the need for carbon addition during antibiotic exposure. Metagenomic analysis revealed that even when inocula were dominated by Pseudomonas, the relative abundance of Stenotrophomonas increased upon biofilm development to ultimately become the dominant species post-streptomycin exposure. The combined metabolic and metagenomic results demonstrated the relevance of inter-species influence on survival and that nutritional status has a strong influence on the survival of P. aeruginosa dominated biofilms.en_ZA
dc.description.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02730/full
dc.description.versionPublisher's version
dc.format.extent11 pages
dc.identifier.citationJackson, L. D. M., et al. 2021. Species interaction and selective carbon addition during antibiotic exposure enhances bacterial survival. Frontiers in Microbiology, 10:2730, doi:10.3389/fmicb.2019.02730
dc.identifier.issn1664-302X (online)
dc.identifier.otherdoi:10.3389/fmicb.2019.02730
dc.identifier.urihttp://hdl.handle.net/10019.1/123350
dc.language.isoen_ZAen_ZA
dc.publisherFrontiers Media
dc.rights.holderAuthors retain copyright
dc.subjectBacteriaen_ZA
dc.subjectBiofilms -- Environmental aspectsen_ZA
dc.subjectPseudomonas aeruginosa -- Analysisen_ZA
dc.subjectStenotrophomonas maltophilia -- Analysisen_ZA
dc.subjectAntibiotics in nutritionen_ZA
dc.subjectBiofilms -- Analysisen_ZA
dc.titleSpecies interaction and selective carbon addition during antibiotic exposure enhances bacterial survivalen_ZA
dc.typeArticleen_ZA
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