Classification of 'Granny Smith' apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis

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dc.contributor.authorMditshwa, Asandaen_ZA
dc.contributor.authorFawole, Olaniyi A.en_ZA
dc.contributor.authorVries, Filicityen_ZA
dc.contributor.authorVan der Merwe, Kobusen_ZA
dc.contributor.authorCrouch, Elkeen_ZA
dc.contributor.authorOpara, Umezuruike Linusen_ZA
dc.date.accessioned2017-06-09T12:24:14Z
dc.date.available2017-06-09T12:24:14Z
dc.date.issued2016
dc.descriptionCITATION: Mditshwa, A. et al. 2016. Classification of 'Granny Smith' apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis. Journal of Applied Botany and Food Quality, 89:49-55, doi:10.5073/JABFQ.2016.089.006.
dc.descriptionThe original publication is available at https://ojs.openagrar.de/index.php/JABFQ
dc.description.abstractTo study the metabolic changes in ‘Granny Smith’ apples with different severities of superficial scald, fruit were stored in normal refrigerated air (0°C, 95% RH) for 12 weeks followed by 7 d shelf-life under room conditions (20°C, 65% RH). Fruit were graded to five groups based on scald severity and analysed for ethylene, α-farnesene and 6-methyl-5-hepten-2-one (MHO) levels. Reactive oxygen species (ROS) were measured by confocal laser-scanning microscopy on apple peel treated with fluorescent probe 2’,7’-dichlorodihydrofluorescein diacetate. Ethylene production rate, α-farnesene and MHO contents and ROS intensity increased with increasing scald severity but declined in severely scalded fruit. Malondialdehyde (MDA) concentration in fruit peel, a measure of membrane damage, increased linearly (R=0.891) with increase in scald severity. Discriminant analysis was used to classify fruit by scald severity on the basis of metabolites accumulated. The stepwise model indicated that three attributes (ROS, ethylene production and MDA) contributed significantly (R2≥0.5) to the separation of the five scald severity indexes, with ROS having the highest contribution (partial R² =0.961; p<0.0001), followed by ethylene (R2=0.718; p<0.0001) and MDA (R2=0.578; p<0.0001).en_ZA
dc.description.urihttps://ojs.openagrar.de/index.php/JABFQ/article/view/4589
dc.description.versionPublisher's version
dc.format.extent7 pages
dc.identifier.citationMditshwa, A. et al. 2016. Classification of 'Granny Smith' apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysis. Journal of Applied Botany and Food Quality, 89:49-55, doi:10.5073/JABFQ.2016.089.006.
dc.identifier.issn1439-040X (online)
dc.identifier.issn1613-9216 (print)
dc.identifier.otherdoi:10.5073/JABFQ.2016.089.006
dc.identifier.urihttp://hdl.handle.net/10019.1/101738
dc.language.isoen_ZAen_ZA
dc.publisherGerman Botanical Society
dc.rights.holderAuthors retain copyright
dc.subjectApplesen_ZA
dc.subjectApples -- Varietiesen_ZA
dc.subjectEthyleneen_ZA
dc.titleClassification of 'Granny Smith' apples with different levels of superficial scald severity based on targeted metabolites and discriminant analysisen_ZA
dc.typeArticleen_ZA
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