Centella asiatica modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s)
| dc.contributor.author | Naidoo, Dhaneshree Bestinee | en_ZA |
| dc.contributor.author | Chuturgoon, Anil Amichund | en_ZA |
| dc.contributor.author | Phulukdaree, Alisa | en_ZA |
| dc.contributor.author | Guruprasad, Kanive Parashiva | en_ZA |
| dc.contributor.author | Satyamoorthy, Kapaettu | en_ZA |
| dc.contributor.author | Sewram, Vikash | en_ZA |
| dc.date.accessioned | 2017-08-07T05:48:25Z | |
| dc.date.available | 2017-08-07T05:48:25Z | |
| dc.date.issued | 2017-08-01 | |
| dc.date.updated | 2017-08-06T03:33:35Z | |
| dc.description | CITATION: Naidoo, D. B. et al. 2017. Centella asiatica modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 17:377, doi:10.1186/s12906-017-1865-2. | |
| dc.description | The original publication is available at https://bmccomplementalternmed.biomedcentral.com | |
| dc.description.abstract | Background: Cancer cachexia is associated with increased pro-inflammatory cytokine levels. Centella asiatica (C. asiatica) possesses antioxidant, anti-inflammatory and anti-tumour potential. We investigated the modulation of antioxidants, cytokines and cell death by C. asiatica ethanolic leaf extract (CLE) in leukaemic THP-1 cells and normal peripheral blood mononuclear cells (PBMC’s). Methods: Cytotoxcity of CLE was determined at 24 and 72 h (h). Oxidant scavenging activity of CLE was evaluated using the 2, 2-diphenyl-1 picrylhydrazyl (DPPH) assay. Glutathione (GSH) levels, caspase (−8, −9, −3/7) activities and adenosine triphosphate (ATP) levels (Luminometry) were then assayed. The levels of tumour necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β and IL-10 were also assessed using enzyme-linked immunosorbant assay. Results: CLE decreased PBMC viability between 33.25–74.55% (24 h: 0.2–0.8 mg/ml CLE and 72 h: 0.4–0.8 mg/ml CLE) and THP-1 viability by 28.404% (72 h: 0.8 mg/ml CLE) (p < 0.0001). Oxidant scavenging activity was increased by CLE (0.05–0.8 mg/ml) (p < 0.0001). PBMC TNF-α and IL-10 levels were decreased by CLE (0.05–0.8 mg/ml) (p < 0.0001). However, PBMC IL-6 and IL-1β concentrations were increased at 0.05–0.2 mg/ml CLE but decreased at 0.4 mg/ml CLE (p < 0.0001). In THP-1 cells, CLE (0.2–0.8 mg/ml) decreased IL-1β and IL-6 whereas increased IL-10 levels (p < 0.0001). In both cell lines, CLE (0.05–0.2 mg/ml, 24 and 72 h) increased GSH concentrations (p < 0.0001). At 24 h, caspase (−9, −3/7) activities was increased by CLE (0.05–0.8 mg/ml) in PBMC’s whereas decreased by CLE (0.2–0.4 mg/ml) in THP-1 cells (p < 0.0001). At 72 h, CLE (0.05–0.8 mg/ml) decreased caspase (−9, −3/7) activities and ATP levels in both cell lines (p < 0.0001). Conclusion: In PBMC’s and THP-1 cells, CLE proved to effectively modulate antioxidant activity, inflammatory cytokines and cell death. In THP-1 cells, CLE decreased pro-inflammatory cytokine levels whereas it increased anti-inflammatory cytokine levels which may alleviate cancer cachexia. | |
| dc.description.uri | https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-017-1865-2 | |
| dc.description.version | Publisher's version | |
| dc.format.extent | 11 pages | |
| dc.identifier.citation | Naidoo, D. B. et al. 2017. Centella asiatica modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s). BMC Complementary and Alternative Medicine, 17:377, doi:10.1186/s12906-017-1865-2. | |
| dc.identifier.issn | 1472-6882 | |
| dc.identifier.other | doi:10.1186/s12906-017-1865-2 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/102055 | |
| dc.language.iso | en | |
| dc.publisher | BioMed Central | |
| dc.rights.holder | Authors retain copyright | |
| dc.subject | Centella asiatica | en_ZA |
| dc.subject | Cytokines | en_ZA |
| dc.subject | Cachexia | en_ZA |
| dc.subject | Cancer | en_ZA |
| dc.title | Centella asiatica modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC’s) | en_ZA |
| dc.type | Article |