The role of paternal chromosomes and sperm morphology on the outcome of intracytoplasmic sperm injection

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dc.contributor.authorRaja K.
dc.contributor.authorFranken D.R.
dc.date.accessioned2011-05-15T16:15:24Z
dc.date.available2011-05-15T16:15:24Z
dc.date.issued2006
dc.description.abstractThe study investigated the possible relationship between the X/Y chromosomes bearing spermatozoa and the outcome of intracytoplasmic sperm injection (ICSI) therapy and morphological appearance/shape (Tygerberg criteria) of the sperm cell injected into the oocyte during ICSI therapy and fertilisation. Thirty-nine patients were recruited for the study from an assisted reproductive programme. Semen samples were prepared by using gradient centrifugation techniques. Prior to injection sperm images were captured using high-quality video graphic equipment. Sperm selection was based on the concept of 'best-looking' spermatozoa i.e. spermatozoa lacking gross and obvious malformations such as broken necks, cytoplasmic droplets, amorphous or elongated heads. Photomicrographs of each sperm cell were produced from video footage. The photographical material was used to determine the basic shape and the actual length-to-width ratio of the injected sperm heads. Embryo biopsies and fluorescent in situ hybridisation (FISH) was performed on 12 randomly selected couples from a set of 39. Embryos were evaluated on day 3 for development and embryo transfer. Embryo biopsies and FISH analyses were performed on those embryos that showed no developmental potential following injection. It was found that 70% of the embryos that showed no developmental potential were Y chromosome-bearing embryos. The sperm selection process for ICSI based on the approach of choosing the 'best-looking' spermatozoon in the ejaculate seems to provide cells that can be classified as normal based on the length-to-width ratio set by the World Health Organization for normal cells. © 2006 The Authors.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation38
dc.identifier.citation5
dc.identifier.issn03034569
dc.identifier.other10.1111/j.1439-0272.2006.00736.x
dc.identifier.urihttp://hdl.handle.net/10019.1/13326
dc.subjectarticle
dc.subjectcentrifugation
dc.subjectchromosome
dc.subjectcontrolled study
dc.subjectcytoplasm
dc.subjectembryo
dc.subjectembryo transfer
dc.subjectfemale
dc.subjectfertilization in vitro
dc.subjectfluorescence in situ hybridization
dc.subjecthuman
dc.subjecthuman cell
dc.subjectintracytoplasmic sperm injection
dc.subjectmale
dc.subjectmicrophotography
dc.subjectmorphology
dc.subjectmorphometrics
dc.subjectoocyte
dc.subjectpaternity
dc.subjectpregnancy
dc.subjectsperm
dc.subjectspermatozoon
dc.subjectspermatozoon abnormality
dc.subjecttreatment outcome
dc.subjectvideorecording
dc.subjectX chromosome
dc.subjectY chromosome
dc.subjectBiopsy
dc.subjectChromosomes, Human, X
dc.subjectChromosomes, Human, Y
dc.subjectEmbryo
dc.subjectEmbryo Transfer
dc.subjectEmbryonic Development
dc.subjectFathers
dc.subjectFemale
dc.subjectHumans
dc.subjectIn Situ Hybridization, Fluorescence
dc.subjectMale
dc.subjectPregnancy
dc.subjectSperm Injections, Intracytoplasmic
dc.subjectSpermatozoa
dc.subjectTreatment Outcome
dc.titleThe role of paternal chromosomes and sperm morphology on the outcome of intracytoplasmic sperm injection
dc.typeArticle
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