Critical assessment of the application of multidetection SEC and AF4 for the separation of single-chain nanoparticles

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dc.contributor.authorEngelke, Johannaen_ZA
dc.contributor.authorBoye, Susanneen_ZA
dc.contributor.authorTuten, Bryan T.en_ZA
dc.contributor.authorBarner, Leonieen_ZA
dc.contributor.authorBarner-Kowollik, Christopheren_ZA
dc.contributor.authorLederer, Albenaen_ZA
dc.date.accessioned2022-05-23T08:00:37Zen_ZA
dc.date.available2022-05-23T08:00:37Zen_ZA
dc.date.issued2020-10en_ZA
dc.descriptionCITATION: Engelke, J. et al. 2020. Critical assessment of the application of multidetection SEC and AF4 for the separation of single-chain nanoparticles. ACS Macro Letters, 9(11):1569-1575. doi:10.1021/acsmacrolett.0c00519en_ZA
dc.descriptionThe original publication is available at https://pubs.acs.org/journal/amlccden_ZA
dc.description.abstractThe intramolecular chain collapse of linear precursors with systematic variation of molar mass and ligation group density (5, 15, and 30 mol %) into single-chain nanoparticles (SCNPs) was studied by two different separation approaches. The efficiency of size exclusion chromatography with quadruple detection (SEC-D4) was compared to asymmetrical field flow fractionation hyphenated to quintuple detection (AF4-D5) in organic solvent. The application of the unique combination of advanced detection to different separation principles opens up the opportunity to critically evaluate the determination of molar masses and different types of radii for an in-depth understanding of the structural properties affected by the internal folding process. This is achieved by a detailed comparison of assets, drawbacks, and limitations of these approaches based on the systematical screening of different chain lengths and sizes of the precursors and the SCNPs. Furthermore, an alternative strategy for quantitative determination of intramolecular ligation density by a combination of AF4 and UV detection is introduced.en_ZA
dc.description.urihttps://pubs.acs.org/doi/10.1021/acsmacrolett.0c00519en_ZA
dc.description.versionPublisher’s versionen_ZA
dc.format.extent7 pagesen_ZA
dc.identifier.citationEngelke, J. et al. 2020. Critical assessment of the application of multidetection SEC and AF4 for the separation of single-chain nanoparticles. ACS Macro Letters, 9(11):1569-1575. doi:10.1021/acsmacrolett.0c00519en_ZA
dc.identifier.issn2161-1653 (online)en_ZA
dc.identifier.otherdoi:10.1021/acsmacrolett.0c00519en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/125228en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherAmerican Chemical Societyen_ZA
dc.rights.holderAmerican Chemical Societyen_ZA
dc.subjectPolymerizationen_ZA
dc.subjectMacromoleculesen_ZA
dc.subjectNanostructured materialsen_ZA
dc.subjectSize exclusion chromatographyen_ZA
dc.titleCritical assessment of the application of multidetection SEC and AF4 for the separation of single-chain nanoparticlesen_ZA
dc.typeArticleen_ZA
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