Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives

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dc.contributor.authorMalherbe, Christiaan J.en_ZA
dc.contributor.authorDe Beer, Daleneen_ZA
dc.contributor.authorJoubert, Elizabethen_ZA
dc.date.accessioned2013-07-03T08:19:46Z
dc.date.available2013-07-03T08:19:46Z
dc.date.issued2012
dc.descriptionCITATION: Malherbe, C. J., De Beer, D. & Joubert, E. 2012. Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives. International Journal of Molecular Sciences, 13(3), doi:10.3390/ijms13033101.
dc.descriptionThe original publication is available at https://www.mdpi.com/1422-0067/13/3/3101
dc.description.abstractBiochemical detection (BCD) methods are commonly used to screen plant extracts for specific biological activities in batch assays. Traditionally, bioactives in the most active extracts were identified through time-consuming bio-assay guided fractionation until single active compounds could be isolated. Not only are isolation procedures often tedious, but they could also lead to artifact formation. On-line coupling of BCD assays to high performance liquid chromatography (HPLC) is gaining ground as a high resolution screening technique to overcome problems associated with pre-isolation by measuring the effects of compounds post-column directly after separation. To date, several on-line HPLC-BCD assays, applied to whole plant extracts and mixtures, have been published. In this review the focus will fall on enzyme-based, receptor-based and antioxidant assays.
dc.description.urihttps://www.mdpi.com/1422-0067/13/3/3101
dc.description.versionPublisher's version
dc.format.extent33 pages
dc.identifier.citationMalherbe, C. J., De Beer, D. & Joubert, E. 2012. Development of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactives. International Journal of Molecular Sciences, 13(3), doi:10.3390/ijms13033101.
dc.identifier.urihttp://hdl.handle.net/10019.1/82665
dc.language.isoen
dc.publisherMDPI
dc.rights.holderAuthors retain copyright
dc.subject.otherBiochemical detection methodsen_ZA
dc.titleDevelopment of on-line high performance liquid chromatography (HPLC)-biochemical detection methods as tools in the identification of bioactivesen_ZA
dc.typeArticle
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