Ultrastructural evaluation of recurrent and in-vitro maturation resistant metaphase I arrested oocytes

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dc.contributor.authorWindt M.-L.
dc.contributor.authorCoetzee K.
dc.contributor.authorKruger T.F.
dc.contributor.authorMarino H.
dc.contributor.authorKitshoff M.S.
dc.contributor.authorSousa M.
dc.date.accessioned2011-05-15T16:16:33Z
dc.date.available2011-05-15T16:16:33Z
dc.date.issued2001
dc.description.abstractAn infertile couple whose female partner showed recurrent retrieval of immature metaphase I (MI) oocytes that were resistant to in-vitro maturation, was studied. Four spermiograms revealed teratozoospermia. Consistent non-fertilization and negative pregnancy outcomes were obtained after intrauterine insemination, gamete intra-Fallopian transfer and IVF. Two intracytoplasmic sperm injection (ICSI) cycles were finally performed. All oocytes (n = 17) in both cycles were arrested at MI and failed to mature after 48 h culture. ICSI also resulted in total non-fertilization. In the last cycle, two oocytes were analysed by transmission electron microscopy and showed almost identical results. All organelles showed normal characteristics of an MI oocyte. The main abnormality found was related to the MI spindle, with absence of microtubules and dispersion of the female chromosomes. Minor abnormalities were observed (immature fibrous appearance of the zona pellucida; the presence of small vesicle aggregates which formed a foam-like body). The injected sperm nucleus was arrested in the middle of the chromatin decondensation process, with no visible nuclear envelope reformation. Normal disruption of sperm acrosomal and flagellar components were observed. Only a partial cortical reaction was observed. This represents the first documented case of developmental arrest due to complete absence of spindle formation in association with an otherwise mature ooplasm.
dc.description.versionArticle
dc.identifier.citationHuman Reproduction
dc.identifier.citation16
dc.identifier.citation11
dc.identifier.issn02681161
dc.identifier.urihttp://hdl.handle.net/10019.1/13834
dc.subjectbromocriptine mesilate
dc.subjectchorionic gonadotropin
dc.subjecthuman menopausal gonadotropin
dc.subjectnafarelin acetate
dc.subjecttriptorelin
dc.subjectacrosome
dc.subjectadult
dc.subjectarticle
dc.subjectcase report
dc.subjectcell aggregation
dc.subjectcell culture
dc.subjectcell growth
dc.subjectcell nucleus
dc.subjectcell nucleus membrane
dc.subjectcell organelle
dc.subjectcell ultrastructure
dc.subjectcell vacuole
dc.subjectchromatin condensation
dc.subjectchromosome segregation
dc.subjectfemale
dc.subjectfertilization
dc.subjectfertilization in vitro
dc.subjectgamete intrafallopian transfer
dc.subjecthuman
dc.subjecthuman cell
dc.subjectinfertility
dc.subjectintracytoplasmic sperm injection
dc.subjectintrauterine insemination
dc.subjectmale
dc.subjectmeiosis
dc.subjectmetaphase
dc.subjectmicrotubule
dc.subjectmitosis spindle
dc.subjectoocyte maturation
dc.subjectpregnancy
dc.subjectspermatozoon tail
dc.subjectspermiogram
dc.subjecttransmission electron microscopy
dc.subjectzona pellucida
dc.subjectAcrosome
dc.subjectChromatin
dc.subjectChromosomes
dc.subjectCulture Techniques
dc.subjectCytoplasm
dc.subjectFemale
dc.subjectFertilization in Vitro
dc.subjectGamete Intrafallopian Transfer
dc.subjectHumans
dc.subjectInfertility
dc.subjectInsemination, Artificial, Homologous
dc.subjectMale
dc.subjectMeiosis
dc.subjectMetaphase
dc.subjectMicroscopy, Electron
dc.subjectMicrotubules
dc.subjectNuclear Envelope
dc.subjectOligospermia
dc.subjectOocytes
dc.subjectSperm Injections, Intracytoplasmic
dc.subjectSperm Tail
dc.subjectSpermatozoa
dc.subjectTreatment Outcome
dc.subjectZona Pellucida
dc.titleUltrastructural evaluation of recurrent and in-vitro maturation resistant metaphase I arrested oocytes
dc.typeArticle
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