Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up

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dc.contributor.advisorSwart, Pieteren_ZA
dc.contributor.advisorGraz, Michaelen_ZA
dc.contributor.authorDe Wet, Tinus Andreen_ZA
dc.contributor.otherStellenbosch University. Faculty of Science. Dept. of Biochemistry.en_ZA
dc.date.accessioned2012-12-01T16:26:10Zen_ZA
dc.date.accessioned2012-12-12T08:11:28Z
dc.date.available2012-12-01T16:26:10Zen_ZA
dc.date.available2012-12-12T08:11:28Z
dc.date.issued2012-12en_ZA
dc.descriptionThesis (MSc)--Stellenbosch University, 2012.en_ZA
dc.description.abstractENGLISH ABSTRACT: This study describes: a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin b) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating: i. Extraction methods ii. Centrifugation iii. Ultrafiltration iv. Chymotrypsinogen and trypsin crystallization v. Column chromatography vi. Investigation into different raw material sources for pancreatic enzyme production c) Development of kinetic and ELISA testing methodologies for in-process QC analysis.en_ZA
dc.description.abstractAFRIKAANSE OPSOMMING: Hierdie Studie beskryf: a) Karakterisering van die ou prosessering metodes en toets metodes wat gebruik word om Tripsien en Alpha-chimotripsien te suiwer en te kwantifiseer. b) Herontwerp / ontwikkeling van 'n nuwe metode vir die suiwering Tripsien en Chimotripsien wat „n hoër opbrengs lewer en meer kontrole oor die proses uit oefen deur ondersoek in te stel na: i. Ekstraksie- metodes ii. Sentrifugering iii. Ultrafiltrasie iv. Chymotripsienogeen - en tripsien kristallisasie v. Kolom chromatografie vi. Ondersoek na verskillende rou materiaal bronne vir die produksie van pankreas ensieme. c) Die ontwikkeling van kinetiese- en ELISA toets metodes vir die in-proses kwaliteitkontrole.af_ZA
dc.format.extent199 p. : col. ill.
dc.identifier.urihttp://hdl.handle.net/10019.1/71790
dc.language.isoen_ZAen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch University
dc.subjectProteins -- Purificationen_ZA
dc.subjectTrypsinen_ZA
dc.subjectProteolytic enzymesen_ZA
dc.subjectDissertations -- Biochemistryen_ZA
dc.subjectTheses -- Biochemistryen_ZA
dc.subject.otherBiochemistryen_ZA
dc.titleLaboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale upen_ZA
dc.typeThesisen_ZA
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