Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa

Simple item page
dc.contributor.authorSnyman, Yolandien_ZA
dc.contributor.authorWhitelaw, Andrew Christopheren_ZA
dc.contributor.authorReuter, Sandraen_ZA
dc.contributor.authorDramowski, Angelaen_ZA
dc.contributor.authorMaloba, Motlatji Reratilwe Bonnieen_ZA
dc.contributor.authorNewton-Foot, Maeen_ZA
dc.date.accessioned2021-08-19T12:12:12Z
dc.date.available2021-08-19T12:12:12Z
dc.date.issued2020
dc.descriptionCITATION: Snyman, Y., et al. 2020. Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. International Journal of Infectious Diseases, 91:94-100, doi:10.1016/j.ijid.2019.11.021.
dc.descriptionThe original publication is available at https://www.journals.elsevier.com/international-journal-of-infectious-diseases
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund
dc.description.abstractObjectives: To describe colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. Methods: A. baumannii isolates identified on Vitek 2 Advanced Expert System were collected from Tygerberg Hospital referral laboratory between 2016 and 2017. Colistin resistance was confirmed using broth microdilution and SensiTest. mcr-1–5 were detected using PCR and strain typing was performed by rep-PCR. Whole genome sequencing (WGS) was performed on a subset of isolates to identify chromosomal colistin resistance mechanisms and strain diversity using multilocus sequence typing (MLST) and pairwise single nucleotide polymorphism analyses. Results: Twenty-six colistin-resistant and six colistin-susceptible A. baumannii were collected separately based on Vitek susceptibility; 20/26 (77%) were confirmed colistin-resistant by broth microdilution. Four colistin-resistant isolates were isolated in 2016 and 16 in 2017, from five healthcare facilities. Thirteen colistin-resistant isolates and eight colistin-susceptible isolates were identical by rep-PCR and MLST (ST1), all from patients admitted to a tertiary hospital during 2017. The remaining colistin-resistant isolates were unrelated. Conclusions: An increase in colistin-resistant A. baumannii isolates from a tertiary hospital in 2017 appears to be clonal expansion of an emerging colistin-resistant strain. This strain was not detected in 2016 or from other hospitals. Identical colistin-susceptible isolates were also isolated, suggesting relatively recent acquisition of colistin resistance.en_ZA
dc.description.urihttps://www.clinicalkey.com/#!/content/playContent/1-s2.0-S1201971219304606?returnurl=https:%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS1201971219304606%3Fshowall%3Dtrue&referrer=https:%2F%2Fpubmed.ncbi.nlm.nih.gov%2F
dc.description.versionPublisher's version
dc.format.extent7 pagesen_ZA
dc.identifier.citationSnyman, Y., et al. 2020. Clonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africa. International Journal of Infectious Diseases, 91:94-100, doi:10.1016/j.ijid.2019.11.021
dc.identifier.issn1201-9712 (online)
dc.identifier.otherdoi:10.1016/j.ijid.2019.11.021
dc.identifier.urihttp://hdl.handle.net/10019.1/110892
dc.language.isoen_ZAen_ZA
dc.publisherElsevier
dc.rights.holderAuthors retain copyright
dc.subjectColistin resistanceen_ZA
dc.subjectAcinetobacteren_ZA
dc.subjectAntibioticsen_ZA
dc.subjectMultidrug resistanceen_ZA
dc.subjectGram-negative bacteriaen_ZA
dc.titleClonal expansion of colistin-resistant Acinetobacter baumannii isolates in Cape Town, South Africaen_ZA
dc.typeArticleen_ZA
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
snyman_clonal_2020.pdf
Size:
1023.77 KB
Format:
Adobe Portable Document Format
Description:
Download article
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Research Articles (Medical Microbiology)