The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay

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dc.contributor.authorHabte, Habtom H.
dc.contributor.authorMall, Anwar S.
dc.contributor.authorDe Beer, Corena
dc.contributor.authorLotz, Zoe E.
dc.contributor.authorKahn, Delawir
dc.date.accessioned2010-12-14T13:42:19Z
dc.date.available2010-12-14T13:42:19Z
dc.date.issued2006-11
dc.date.updated2010-11-09T12:58:41Z
dc.descriptionIncludes bibliography
dc.description.abstractBackground: Despite the continuous shedding of HIV infected blood into the oral cavity and the detectable presence of the AIDS virus at a high frequency, human saliva is reported to inhibit oral transmission of HIV through kissing, dental treatment, biting, and aerosolization. The purpose of this study was to purify salivary MUC5B and MUC7 mucins from crude saliva and determine their anti-HIV-1 activities. Methods: Following Sepharose CL-4B column chromatography and caesium chloride isopycnic density-gradient ultra-centrifugation, the purity and identity of the mucins was determined by SDS-PAGE and Western blotting analysis respectively. Subsequently an HIV-1 inhibition assay was carried out to determine the anti-HIV-1 activity of the crude saliva and purified salivary mucins by incubating them with subtype D HIV-1 prior to infection of the CD4+ CEM SS cells. Results: Western blotting analysis confirmed that the mucin in the void volume is MUC5B and the mucin in the included volume is MUC7. The HIV inhibition assay revealed that both the crude saliva and salivary MUC5B and MUC7 mucins inhibited HIV-1 activity by 100%. Conclusion: Although the mechanism of action is not clear the carbohydrate moieties of the salivary mucins may trap or aggregate the virus and prevent host cell entry.en_ZA
dc.description.versionPeer Reviewed
dc.format.extent12 p. : col. ill.
dc.identifier.citationHabte, HH, Mall, AS, De Beer, C, Lotz, ZE & Kahn, D 2006, 'The role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assay', Virology Journal, 3(1):99.en_ZA
dc.identifier.issn1743-422X
dc.identifier.otherhttp://dx.doi.org/10.1186/1743-422X-3-99
dc.identifier.urihttp://hdl.handle.net/10019.1/5119
dc.language.isoen_ZAen_ZA
dc.language.rfc3066en
dc.publisherBioMed Centralen_ZA
dc.rights.holderHabte et al.; licensee BioMed Central Ltd.en_ZA
dc.subjectHuman salivaen_ZA
dc.subjectHIV transmissionen_ZA
dc.subjectHIV (Viruses) -- Oral transmission -- Researchen_ZA
dc.titleThe role of crude human saliva and purified salivary MUC5B and MUC7 mucins in the inhibition of Human Immunodeficiency Virus type 1 in an inhibition assayen_ZA
dc.typeArticleen_ZA
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