Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes

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dc.contributor.authorClarke, Charleneen_ZA
dc.contributor.authorSmith, Katrinen_ZA
dc.contributor.authorGoldswain, Samantha J.en_ZA
dc.contributor.authorHelm, Christopheren_ZA
dc.contributor.authorCooper, David V.en_ZA
dc.contributor.authorKerr, Tanya J.en_ZA
dc.contributor.authorKleynhans, Leanieen_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.contributor.authorMiller, Michele A.en_ZA
dc.contributor.authorGoosen, Wynand J.en_ZA
dc.date.accessioned2021-04-12T09:49:39Z
dc.date.available2021-04-12T09:49:39Z
dc.date.issued2021
dc.descriptionCITATION: Clarke, C., et al. 2021. Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes. Scientific Reports, 11:7061, doi:10.1038/s41598-021-86682-5
dc.descriptionThe original publication is available at https://www.nature.com
dc.descriptionPublication of this article was funded by the Stellenbosch University Open Access Fund
dc.description.abstractENGLISH ABSTRACT: Mycobacterium bovis is the causative agent of bovine tuberculosis (bTB) in wildlife. Confirmation of M. bovis infection relies on mycobacterial culture, which is time-consuming. Collection and transportation of infectious material also pose a human health risk. PrimeStore Molecular Transport Medium (MTM) has been shown to effectively inactivate infectious organisms, making it a safe method for handling infectious samples. This study investigated an in-field sampling technique for rapid, safe detection of M. bovis in buffalo tissues. Potentially infected tissues from bTB test-positive buffaloes were swabbed at post-mortem examination and stored in PrimeStore MTM at ambient temperature until Xpert MTB/RIF Ultra testing was performed. Additionally, tissue samples were frozen and transported before homogenisation for culture and Ultra testing. Oral swabs were collected from M. bovis-unexposed buffaloes as a negative control cohort. Mycobacterium tuberculosis complex (MTBC) DNA was detected by Ultra in 13/16 tissue swabs and 9/16 matched tissue homogenates from culture-confirmed M. bovis-positive buffalo tissues. MTBC DNA was not detected in swabs from M. bovis-unexposed animals, showing the potentially high specificity of Ultra with PrimeStore swabs. PrimeStore MTM sample processing, in combination with the Ultra assay, has the potential to provide a safe, rapid post-mortem screening test for M. bovis in buffaloes.en_ZA
dc.description.urihttps://www.nature.com/articles/s41598-021-86682-5
dc.description.versionPublisher's version
dc.format.extent6 pagesen_ZA
dc.identifier.citationClarke, C., et al. 2021. Novel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloes. Scientific Reports, 11:7061, doi:10.1038/s41598-021-86682-5
dc.identifier.issn2045-2322 (online)
dc.identifier.otherdoi:10.1038/s41598-021-86682-5
dc.identifier.urihttp://hdl.handle.net/10019.1/109749
dc.language.isoen_ZAen_ZA
dc.publisherNature Research (part of Springer Nature)en_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMycobacterium bovisen_ZA
dc.subjectZoonosesen_ZA
dc.subjectAfrican buffalo -- Communicable diseases -- Transmissionen_ZA
dc.titleNovel molecular transport medium used in combination with Xpert MTB/RIF ultra provides rapid detection of Mycobacterium bovis in African buffaloesen_ZA
dc.typeArticleen_ZA
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