The influence of homogenous zona pellucida on human spermatozoa hyperactivation, acrosome reaction and zona binding

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dc.contributor.authorBastiaan H.
dc.contributor.authorFranken D.
dc.date.accessioned2011-05-15T16:15:24Z
dc.date.available2011-05-15T16:15:24Z
dc.date.issued2007
dc.description.abstractThe study aimed to evaluate the changes in sperm motion characteristics and the occurrence of hyperactivation among sperm populations after exposure to human zona pellucida. Motile spermatozoa samples were used to evaluate the sperm-zona binding capacity, zona-induced acrosome reaction and changes in sperm motion characteristics. Sperm motion characteristic changes studied included straight line velocity, curvilinear velocity, amplitude of lateral head displacement, straightness and beat cross frequency. Recordings were performed on semen immediately after liquefaction, 3 h capacitation and after exposure to solubilised human zona pellucida. The semen samples were divided into morphology categories, namely six (16 ± 1.4% normal forms, normal patterns), 31 (8 ± 1.7% normal forms, G-pattern) and 27 (3 ± 1.3% normal forms, P-pattern). The Hemizona Indices for the three morphology groups namely normal, G-patterns and P-patterns, were 77 ± 6%, 61 ± 5% and 41 ± 5% respectively (P ≤ 0.05). The zona-induced acrosome reaction for the three groups were 25.0 ± 2% (normal group), 18.0 ± 2% (G-patterns) and 14.1 ± 0.6% (P-pattern) (P ≤ 0.05). The number of hyperactivated sperm showed an increase in the ejaculates (1%), after capacitation (2%) and exposure to zona pellucida (5%) (P ≤ 0.05). When divided into the three morphology groups, the number of spermatozoa with hyperactivated motility was more profound in men with normal morphology compared with G- and P-pattern men. Human zona pellucida is a strong inducer of hyperactivated motility among spermatozoa and the effect is more profound among sperm populations with normal morphology. © 2007 The Authors.
dc.description.versionArticle
dc.identifier.citationAndrologia
dc.identifier.citation39
dc.identifier.citation1
dc.identifier.issn03034569
dc.identifier.other10.1111/j.1439-0272.2006.00751.x
dc.identifier.urihttp://hdl.handle.net/10019.1/13325
dc.subjectacrosome
dc.subjectarticle
dc.subjectcontrolled study
dc.subjecthuman
dc.subjectliquefaction
dc.subjectmale
dc.subjectmorphology
dc.subjectsperm
dc.subjectspermatozoon
dc.subjectspermatozoon capacitation
dc.subjectzona pellucida
dc.subjectAcrosome Reaction
dc.subjectFemale
dc.subjectHumans
dc.subjectMale
dc.subjectSperm Motility
dc.subjectSpermatozoa
dc.subjectZona Pellucida
dc.titleThe influence of homogenous zona pellucida on human spermatozoa hyperactivation, acrosome reaction and zona binding
dc.typeArticle
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