A unifying model for reconstructing radiosensitivity from micronucleus formation, apoptosis and abnormal morphology

Simple item page
dc.contributor.authorAkudugu, J. M.
dc.contributor.authorAbend, M.
dc.contributor.authorBohm, L.
dc.date.accessioned2011-05-15T16:17:47Z
dc.date.available2011-05-15T16:17:47Z
dc.date.issued2002
dc.description.abstractAt present micronucleus data cannot predict cellular radiosensitivity. The inclusion of data from apoptosis and abnormal morphology has not entirely resolved this problem. Here, we assess the probability of cell death arising from events other than micronucleation, apoptosis and abnormal morphology (i.e. lesions not detected by these damage assays) Poe, for its ability to reflect intrinsic cellular radiosensitivity. Analysis of data from 17 cell lines used in two separate studies, spanning a wide range of radiosensitivity (0.09≤SF2≤0.70), confirmed our previous observation that cell death due to undetected lesions depends on the irradiation dose and is cell type-specific. We further demonstrate that Poe accounts for inter-cell line differences in translating irradiation damage into cell death. Data from any two of micronucleus formation, apoptosis and abnormal cell morphology, fitted to the Poe model, adequately predict clonogenic survival, and measurement of additional damage endpoints is not required. The Poe model may benefit patient selection in situations where colony formation of primary tumour cultures fails to arrive at estimates of radiosensitivity.
dc.description.versionArticle
dc.identifier.citationRadiation and Environmental Biophysics
dc.identifier.citation41
dc.identifier.citation4
dc.identifier.issn0301634X
dc.identifier.urihttp://hdl.handle.net/10019.1/14372
dc.subjectapoptosis
dc.subjectarticle
dc.subjectbiological model
dc.subjectcalculation
dc.subjectcancer cell culture
dc.subjectcell clone
dc.subjectcell damage
dc.subjectcell death
dc.subjectcell specificity
dc.subjectcell strain
dc.subjectcell survival
dc.subjectcell type
dc.subjectcell ultrastructure
dc.subjectclonogenic assay
dc.subjectcolony formation
dc.subjectcontrolled study
dc.subjectcorrelation analysis
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmathematical model
dc.subjectmeasurement
dc.subjectmicronucleus
dc.subjectparameter
dc.subjectpatient selection
dc.subjectprediction
dc.subjectradiation dose
dc.subjectradiation injury
dc.subjectradiation response
dc.subjectradiosensitivity
dc.subjectApoptosis
dc.subjectCell Line
dc.subjectCell Survival
dc.subjectDose-Response Relationship, Radiation
dc.subjectHumans
dc.subjectMicronuclei, Chromosome-Defective
dc.subjectMicronucleus Tests
dc.subjectModels, Theoretical
dc.subjectRadiation Tolerance
dc.subjectTime Factors
dc.titleA unifying model for reconstructing radiosensitivity from micronucleus formation, apoptosis and abnormal morphology
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications