Circulating miR-30a-5p and miR-182-5p in prediabetes and screen-detected diabetes mellitus

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dc.contributor.authorWeale, Cecil Jacken_ZA
dc.contributor.authorMatshazi, Don M.en_ZA
dc.contributor.authorDavids, Saarah F. G.en_ZA
dc.contributor.authorRaghubeer, Shanelen_ZA
dc.contributor.authorErasmus, Rajiv T.en_ZA
dc.contributor.authorKengne, Andre Pascalen_ZA
dc.contributor.authorDavison, Glenda Maryen_ZA
dc.contributor.authorMatsha, Tandi E.en_ZA
dc.date.accessioned2022-06-27T07:47:17Zen_ZA
dc.date.available2022-06-27T07:47:17Zen_ZA
dc.date.issued2020-12en_ZA
dc.descriptionCITATION: Weale C. J. et al. 2020. Circulating miR-30a-5p and miR-182-5p in Prediabetes and Screen-Detected Diabetes Mellitus. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13:5037-5047. doi:10.2147/DMSO.S286081en_ZA
dc.descriptionThe original publication is available at https://www.dovepress.com/diabetes-metabolic-syndrome-and-obesity-targets-and-therapy-journalen_ZA
dc.description.abstractBackground: microRNAs (miRNAs) have been touted as potential diagnostic and prognostic biomarkers for various diseases. The aim of the present study was to evaluate the diagnostic value of miR-30a-5p and miR-182-5p for prediabetes and screen-detected type 2 diabetes mellitus (T2DM). Methods: The study included 1270 participants (207 prediabetes, 94 screen-detected diabetes and 969 normotolerant) from the Vascular and Metabolic Health (VMH) study. Whole blood levels of miR-30a-5p and miR-182-5p were quantitated by RT-qPCR. Multivariable logistic regressions were used to relate miRNAs with prediabetes or T2DM and receiver operating characteristic (ROC) curves were used to evaluate the ability of each miRNA to diagnose these conditions. Results: Both miRNAs were significantly highly expressed in individuals with prediabetes or T2DM (both ≥3.2-fold, and p<0.001). We also observed significant under-expression in T2DM relative to prediabetes for miR-182-5p (0.49-fold, p=0.001). Age, sex and BMI-adjusted partial correlation coefficient analysis revealed a significant correlation between the two miRNAs across glucose tolerance statuses (r≥0.932, p<0.001). In normotolerant individuals, both miRNAs showed a negative correlation with waist circumference and positive correlation with HDL-cholesterol whilst in T2DM they correlated positively with hip circumference, 2-hour insulin, HDL- and LDL-cholesterol. Multivariable logistic regressions revealed both miRNAs to be consistently and continuously associated with prediabetes or T2DM (OR≥1.18, 95% 95% CI: 1.10-1.28, p<0.001), while only miR-182-5p associated with a reduced prevalence of T2DM relative to prediabetes (OR: 0.89, 95% CI: 0.83-0.96, p=0.003). In ROC analyses, miR-182-5p almost outperformed HbA1c in diagnosing prediabetes; area under the curve 0.74 vs 0.69. Conclusion: Our findings demonstrate that miR-30a-5p and miR-182-5p are associated with dysglycaemia and could potentially predict prediabetes, particularly miR-182-5p.en_ZA
dc.description.urihttps://www.dovepress.com/circulating-mir-30a-5p-and-mir-182-5p-in-prediabetes-and-screen-detect-peer-reviewed-fulltext-article-DMSOen_ZA
dc.description.versionPublishers versionen_ZA
dc.format.extent11 pagesen_ZA
dc.identifier.citationWeale C. J. et al. Circulating miR-30a-5p and miR-182-5p in Prediabetes and Screen-Detected Diabetes Mellitus. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13:5037-5047. doi:10.2147/DMSO.S286081en_ZA
dc.identifier.issn1178-7007 (print)en_ZA
dc.identifier.otherdoi:10.2147/DMSO.S286081en_ZA
dc.identifier.urihttp://hdl.handle.net/10019.1/125408en_ZA
dc.language.isoen_ZAen_ZA
dc.publisherDove Pressen_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectMicroRNA -- Africaen_ZA
dc.subjectDiabetes mellitusen_ZA
dc.subjectBiochemical markersen_ZA
dc.subjectDiabetes -- Diagnosisen_ZA
dc.subjectPrediabetic stateen_ZA
dc.titleCirculating miR-30a-5p and miR-182-5p in prediabetes and screen-detected diabetes mellitusen_ZA
dc.typeArticleen_ZA
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