Population structure of mixed Mycobacterium tuberculosis infection is strain genotype and culture medium dependent

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dc.contributor.authorHanekom, Madeleineen_ZA
dc.contributor.authorStreicher, Elizabeth M.en_ZA
dc.contributor.authorVan de Berg, Doreenen_ZA
dc.contributor.authorCox, Helenen_ZA
dc.contributor.authorMcDermid, Cherylen_ZA
dc.contributor.authorBosman, Marleinen_ZA
dc.contributor.authorGey van Pittius, Nicolaas C.en_ZA
dc.contributor.authorVictor, Tommie C.en_ZA
dc.contributor.authorKidd, Martinen_ZA
dc.contributor.authorVan Soolingen, Dicken_ZA
dc.contributor.authorVan Helden, Paul D.en_ZA
dc.contributor.authorWarren, Robin M.en_ZA
dc.date.accessioned2014-07-07T12:10:16Z
dc.date.available2014-07-07T12:10:16Z
dc.date.issued2013-07-30
dc.descriptionCITATION: Hanekom, M. et al. 2013. Population structure of mixed Mycobacterium tuberculosis infection is strain genotype and culture medium dependent. PLoS ONE, 8(7): e70178, doi:10.1371/journal.pone.0070178.
dc.descriptionThe original publication is available at http://journals.plos.org
dc.description.abstractBackground Molecular genotyping methods have shown infection with more than one Mycobacterium tuberculosis strain genotype in a single sputum culture, indicating mixed infection. Aim This study aimed to develop a PCR-based genotyping tool to determine the population structure of M. tuberculosis strain genotypes in primary Mycobacterial Growth Indicator Tubes (MGIT) and Löwenstein–Jensen (LJ) cultures to identify mixed infections and to establish whether the growth media influenced the recovery of certain strain genotypes. Method A convenience sample of 206 paired MGIT and LJ M. tuberculosis cultures from pulmonary tuberculosis patients resident in Khayelitsha, South Africa were genotyped using an in-house PCR-based method to detect defined M. tuberculosis strain genotypes. Results The sensitivity and specificity of the PCR-based method for detecting Beijing, Haarlem, S-family, and LAM genotypes was 100%, and 75% and 50% for detecting the Low Copy Clade, respectively. Thirty-one (15%) of the 206 cases showed the presence of more than one M. tuberculosis strain genotype. Strains of the Beijing and Haarlem genotypes were significantly more associated with a mixed infection (on both media) when compared to infections with a single strain (Beijing MGIT p = 0.02; LJ, p<0.01) and (Haarlem: MGIT p<0.01; LJ, p = 0.01). Strains with the Beijing genotype were less likely to be with “other genotype” strains (p<0.01) while LAM, Haarlem, S-family and LCC occurred independently with the Beijing genotype. Conclusion The PCR-based method was able to identify mixed infection in at least 15% of the cases. LJ media was more sensitive in detecting mixed infections than MGIT media, implying that the growth characteristics of M. tuberculosis on different media may influence our ability to detect mixed infections. The Beijing and Haarlem genotypes were more likely to occur in a mixed infection than any of the other genotypes tested suggesting pathogen-pathogen compatibility.
dc.description.urihttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0070178
dc.description.versionPublisher's version
dc.format.extent6 pages
dc.identifier.citationHanekom, M. et al. 2013. Population structure of mixed Mycobacterium tuberculosis infection is strain genotype and culture medium dependent. PLoS ONE, 8(7): e70178, doi:10.1371/journal.pone.0070178.
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0070178
dc.identifier.urihttp://hdl.handle.net/10019.1/91564
dc.language.isoen
dc.publisherPublic Library of Science
dc.rights.holderAuthors retain copyright
dc.subjectMycobacterium tuberculosisen_ZA
dc.titlePopulation structure of mixed Mycobacterium tuberculosis infection is strain genotype and culture medium dependenten_ZA
dc.typeArticle
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