dc.contributor.author	Alexandersson, Erik
dc.contributor.author	Becker, John V. W.
dc.contributor.author	Jacobson, Dan
dc.contributor.author	Nguema-Ona, Eric
dc.contributor.author	Steyn, Cobus
dc.contributor.author	Denby, Katherine J.
dc.contributor.author	Vivier, Melane A.
dc.date.accessioned	2011-12-15T08:12:07Z
dc.date.available	2011-12-15T08:12:07Z
dc.date.issued	2011-11
dc.date.updated	2011-12-14T12:07:36Z
dc.description	The original publication is available at http://www.biomedcentral.com/1756-0500/4/493
dc.description.abstract	Abstract: Background Polygalacturonase-inhibiting proteins (PGIPs) directly limit the effective ingress of fungal pathogens by inhibiting cell wall-degrading endopolygalacturonases (ePGs). Transgenic tobacco plants over-expressing grapevine (Vitis vinifera) Vvpgip1 have previously been shown to be resistant to Botrytis infection. In this study we characterized two of these PGIP over-expressing lines with known resistance phenotypes by gene expression and hormone profiling in the absence of pathogen infection. Results Global gene expression was performed by a cross-species microarray approach using a potato cDNA microarray. The degree of potential cross-hybridization between probes was modeled by a novel computational workflow designed in-house. Probe annotations were updated by predicting probe-to-transcript hybridizations and combining information derived from other plant species. Comparing uninfected Vvpgip1-overexpressing lines to wild-type (WT), 318 probes showed significant change in expression. Functional groups of genes involved in metabolism and associated to the cell wall were identified and consequent cell wall analysis revealed increased lignin-levels in the transgenic lines, but no major differences in cell wall-derived polysaccharides. GO enrichment analysis also identified genes responsive to auxin, which was supported by elevated indole-acetic acid (IAA) levels in the transgenic lines. Finally, a down-regulation of xyloglucan endotransglycosylase/hydrolases (XTHs), which are important in cell wall remodeling, was linked to a decrease in total XTH activity. Conclusions This evaluation of PGIP over-expressing plants performed under pathogen-free conditions to exclude the classical PGIP-ePG inhibition interaction indicates additional roles for PGIPs beyond the inhibition of ePGs.	en_ZA
dc.description.version	Publishers' Version	en_ZA
dc.description.version	Under the Creative Commons Attribution license	en_ZA
dc.format.extent	17 p. : col. ill.
dc.identifier.citation	Alexandersson, E. et al 2011. Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco. BMC Research Notes. 4(1):493-509	en_ZA
dc.identifier.uri	http://dx.doi.org/10.1186/1756-0500-4-493
dc.identifier.uri	http://hdl.handle.net/10019.1/18202
dc.language.iso	en_ZA	en_ZA
dc.language.rfc3066	en
dc.publisher	BioMed Central	en_ZA
dc.rights.holder	Alexandersson et al.; licensee BioMed Central Ltd.	en_ZA
dc.subject	Phytopathogenic microorganisms -- Biological control	en_ZA
dc.subject	Tobacco -- Disease and pest resistance	en_ZA
dc.subject	Polygalacturonase	en_ZA
dc.subject	Plant-pathogen relations	en_ZA
dc.title	Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco	en_ZA
dc.type	Article	en_ZA

Constitutive expression of a grapevine polygalacturonase-inhibiting protein affects gene expression and cell wall properties in uninfected tobacco

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