Comparison of motility characteristics and normal sperm morphology of human semen samples separated by Percoll density gradient centrifugation

Simple item page
dc.contributor.authorClaassens O.E.
dc.contributor.authorKaskar K.
dc.contributor.authorCoetzee K.
dc.contributor.authorLombard C.J.
dc.contributor.authorFranken D.R.
dc.contributor.authorKruger T.F.
dc.date.accessioned2011-05-15T16:15:34Z
dc.date.available2011-05-15T16:15:34Z
dc.date.issued1996
dc.description.abstractTile aim of this study was twofold: to investigate the ability of Percoll gradient centrifugation (52, 68, 84%) to fractionate semen samples according to motility quality and percentage normal morphology and to determine whether there is an association between sperm motility quality and percentage normal morphology. Sperm motility was evaluated using a Hamilton Thorn analyzer and normal sperm morphology was manually assessed according to the strict criteria (≤4, 5-14, and >14%). The majority of motility parameters and the percentage normal morphology were found to be significantly improved in the 84% Percoll fraction. The greatest effect was on the 14% group, shifting the mean normal morphology percentage from 2.6 to 5.2%. Curvilinear velocity (VCL) and average path velocity (VAP) were the only two motility parameters that were significantly associated with the percentage normal morphology. Using a combined VCL,VAP vector the >14% group was found to have a significantly different value as compared to the 5-14 and <4% groups. Percoll (discontinuous) gradient centrifugation can therefore play a significant role in the improvement of semen samples for use in assisted reproduction procedures. The VCL,VAP vector identified may also serve as an additional tool in the prediction of the fertility potential of sperm samples.
dc.description.versionArticle
dc.identifier.citationArchives of Andrology
dc.identifier.citation36
dc.identifier.citation2
dc.identifier.issn01485016
dc.identifier.urihttp://hdl.handle.net/10019.1/13396
dc.subjectarticle
dc.subjectcontrolled study
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmale
dc.subjectmorphology
dc.subjectnormal human
dc.subjectpriority journal
dc.subjectspermatozoon motility
dc.subjectAnalysis of Variance
dc.subjectCentrifugation, Density Gradient
dc.subjectColloids
dc.subjectHumans
dc.subjectMale
dc.subjectMicroscopy, Video
dc.subjectPovidone
dc.subjectReference Values
dc.subjectSemen
dc.subjectSilicon Dioxide
dc.subjectSperm Motility
dc.titleComparison of motility characteristics and normal sperm morphology of human semen samples separated by Percoll density gradient centrifugation
dc.typeArticle
Files
Collections
Stellenbosch University - Scopus Tygerberg Hospital Publications