Molecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactives

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dc.contributor.advisorMakunga, Nokwanda
dc.contributor.authorRamogola, Watsie Princess Neo
dc.contributor.otherStellenbosch University. Faculty of AgriSciences. Dept. of Genetics.en_ZA
dc.date.accessioned2012-08-10T17:08:13Z
dc.date.available2012-08-10T17:08:13Z
dc.date.issued2009-12
dc.descriptionThesis (MSc)--Stellenbosch University, 2009.en_ZA
dc.description.abstractENGLISH ABSTRACT: Biotechnological applications are useful for adding value to the local medicinal plants and may provide an alternative source of pharmacologically-active compounds thus assisting with the conservation of wild populations. Salvia africana-lutea aromatic herb has long been used in folk medicine by traditional healers in the Western Cape Province (South Africa) for various ailments. As an in vitro conservation strategy, a continuous S. africana-lutea shoot culture was successfully established in solid MS medium containing BA (0.5 mg L-1) and NAA (0.2 mg L-1). The regeneration rate of the S. africana-lutea plants was high which produced approximately 720 plantlets in 20 culture bottles over a four week cycle. The microshoots were rooted in the MS medium without PGRs prior to acclimatisation. A survival rate of 92% was recorded for the greenhouse-acclimatised shoots.en_ZA
dc.description.abstractAFRIKAANS OPSOMMING: Biotegnologiese toepassings is nuttig vir waarde toevoeging tot inheemse medisinale plante en kan ‘n alternatiewe bron van farmakologies aktiewe verbindings verskaf wat bydrae tot die bewaring van populasies in die natuur. Die aromatiese krui Salvia. Africana-lutea is reeds vir ‘n lang tydperk in volks medisyne deur tradisionele geneesheers in die Wes Kaap provinsie (Suid Afrika) vir ‘n verskeidenheid kwale gebruik. ‘n Kontinu S. africana-lutea lootkultuur in soliede Murashige en Skoog (1962) (MS) media wat BA (0.5 mg L-1) en NAA (0.2 mg L-1) bevat, is suksesvol as ‘n in vitro konservasie strategie ontwikkel. Die regenerasie tempo van die S. africana-lutea plante was hoog en het ongeveer 720 plante in 20 kultuur bottels tydens ‘n vier week siklus gelewer. Die mikrolote is op plant groei reguleerder vrye MS media gewortel voordat plante geaklimatiseer is. ’n Oorlewingstempo van 92% is vir die glashuis geaklimatiseerde lote waargeneem.af_ZA
dc.format.extentxxvi, 160 p. : ill.
dc.identifier.urihttp://hdl.handle.net/10019.1/40563
dc.language.isoen_ZA
dc.publisherStellenbosch : Stellenbosch Universityen_ZA
dc.rights.holderStellenbosch Universityen_ZA
dc.subjectSalvia africana-lutea aromatic herb -- Antimicrobial bioactivityen_ZA
dc.subjectSalvia africana-lutea -- Molecular analysisen_ZA
dc.subjectSalvia africana-lutea -- Medicinal useen_ZA
dc.subjectTheses -- Geneticsen_ZA
dc.subjectDissertations -- Geneticsen_ZA
dc.titleMolecular analyses of Salvia Africana-Lutea L. transgenic hairy root clones for secondary bioactivesen_ZA
dc.typeThesisen_ZA
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