Enzymatic Hydrolysis of Softwood Derived Paper Sludge by an In Vitro Recombinant Cellulase Cocktail for the Production of Fermentable Sugars

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dc.contributor.authorMalgas, Samkeloen_ZA
dc.contributor.authorRose, Shaunita Hen_ZA
dc.contributor.authorvan Zyl, Willem Hen_ZA
dc.contributor.authorPletschke, Brett Ien_ZA
dc.date.accessioned2022-04-29T07:45:56Z
dc.date.available2022-04-29T07:45:56Z
dc.date.issued2020
dc.descriptionCITATION:Malgas S, Rose SH, van Zyl WH, Pletschke BI. Enzymatic Hydrolysis of Softwood Derived Paper Sludge by an In Vitro Recombinant Cellulase Cocktail for the Production of Fermentable Sugars. Catalysts. 2020; 10(7):775. https://doi.org/10.3390/catal10070775
dc.description.abstractAbstract: Paper sludge is an attractive biomass feedstock for bioconversion to ethanol due to its low cost and the lack of pretreatment required for its bioprocessing. This study assessed the use of a recombinant cellulase cocktail (mono-components: S. cerevisiae-derived PcBGL1B (BGL), TeCel7A (CBHI), ClCel6A (CBHII) and TrCel5A (EGII) mono-component cellulase enzymes) for the efficient saccharification of softwood-derived paper sludge to produce fermentable sugars. The paper sludge mainly contained 74.3% moisture and 89.7% (per dry mass (DM)) glucan with a crystallinity index of 91.5%. The optimal protein ratio for paper sludge hydrolysis was observed at 9.4: 30.2: 30.2: 30.2% for BGL: CBHI: CBHII: EGII. At a protein loading of 7.5 mg/g DW paper sludge, the yield from hydrolysis was approximately 80%, based on glucan, with scanning electron microscopy micrographs indicating a significant alteration in the microfibril size (length reduced from ≥ 2 mm to 93 µm) of the paper sludge. The paper sludge hydrolysis potential of the Opt CelMix (formulated cellulase cocktail) was similar to the commercial Cellic CTec2® and Celluclast® 1.5 L cellulase preparations and better than Viscozyme® L. Low enzyme loadings (15 mg/g paper sludge) of the Opt CelMix and solid loadings ranging between 1 to 10% (w/v) rendered over 80% glucan conversion. The high glucose yields attained on the paper sludge by the low enzyme loading of the Opt CelMix demonstrated the value of enzyme cocktail optimisation on specific substrates for efficient cellulose conversion to fermentable sugars.en_ZA
dc.format.extent17 pages
dc.identifier.otherdoi.org/10.3390/catal10070775
dc.identifier.urihttp://hdl.handle.net/10019.1/124486
dc.language.isoen_ZAen_ZA
dc.rights.holderAuthors retain copyright
dc.subjectCellulaseen_ZA
dc.subjectcelluloseen_ZA
dc.subjectPaper Sludgeen_ZA
dc.subjectSaccharomyces cerevisiaeen_ZA
dc.subjectsynergismen_ZA
dc.titleEnzymatic Hydrolysis of Softwood Derived Paper Sludge by an In Vitro Recombinant Cellulase Cocktail for the Production of Fermentable Sugarsen_ZA
dc.typeArticleen_ZA
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