Suitability of saliva for tuberculosis diagnosis : comparing with serum

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dc.contributor.authorNamuganga, Anna R.en_ZA
dc.contributor.authorChegou, Novel N.en_ZA
dc.contributor.authorMubiri, Paulen_ZA
dc.contributor.authorWalzl, Gerharden_ZA
dc.contributor.authorMayanja-Kizza, Harrieten_ZA
dc.date.accessioned2017-09-04T06:39:05Z
dc.date.available2017-09-04T06:39:05Z
dc.date.issued2017-08-31
dc.date.updated2017-09-03T03:28:36Z
dc.descriptionCITATION: Namuganga, A. R., et al. 2017. Suitability of saliva for Tuberculosis diagnosis : comparing with serum. BMC Infectious Diseases, 17:600, doi:10.1186/s12879-017-2687-z.
dc.descriptionThe original publication is available at https://bmcinfectdis.biomedcentral.com
dc.description.abstractBackground In the search for fast, simple and better ways for diagnosis of tuberculosis (TB), there is need to discover and evaluate new biomarkers that are found in samples other than sputum to determine their effectiveness. This study examined the utility of saliva vis-a-vis serum by evaluating levels of biomarkers found in saliva and serum from TB suspects. Methods Study enrolled tuberculosis suspects. Sputum MGIT was used as the gold standard for active TB. Quantiferon gold-In tube assay was done to identify exposure to Mycobacterium tuberculosis (M.tb). Multiplex assay was run for 10 markers using a 10 plex customized kit from Bio-Rad Laboratories. Results There was a significant difference between saliva and serum marker levels. Saliva had significantly higher levels of GM-CSF and VEGF. Serum had higher levels of MIP-1a, b, TNF-a, G-CSF and IFN-g. Serum levels of IL-6, VEGF and TNF-a were significantly different between participants with active TB disease and those with other respiratory diseases. Conclusion Salivary TB biomarkers are worth the search to evaluate their ability to differentiate between TB disease states for generation of a non invasive point of care test for TB diagnosis.
dc.description.urihttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-017-2687-z
dc.description.versionPublisher's version
dc.format.extent11 pages
dc.identifier.citationNamuganga, A. R., et al. 2017. Suitability of saliva for Tuberculosis diagnosis : comparing with serum. BMC Infectious Diseases, 17:600, doi:10.1186/s12879-017-2687-z
dc.identifier.issn1471-2334 (online)
dc.identifier.otherdoi:10.1186/s12879-017-2687-z
dc.identifier.urihttp://hdl.handle.net/10019.1/102167
dc.language.isoen_ZA
dc.publisherBiomed Central
dc.rights.holderAuthors retain copyright
dc.subjectTuberculosis -- Diagnosisen_ZA
dc.subjectSaliva -- Examinationen_ZA
dc.subjectSerodiagnosisen_ZA
dc.titleSuitability of saliva for tuberculosis diagnosis : comparing with serum
dc.typeArticle
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