Phylogeny of Mycobacterium tuberculosis Beijing strains constructed from Polymorphisms in genes involved in DNA replication, recombination and repair

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dc.contributor.authorMestre, Olga
dc.contributor.authorLuo, Tao
dc.contributor.authorDos Vultos, Tiago
dc.contributor.authorKremer, Kristin
dc.contributor.authorMurray, Alan
dc.contributor.authorNamouchi, Amine
dc.contributor.authorJackson, Celine
dc.contributor.authorRauzier, Jean
dc.contributor.authorBifani, Pablo
dc.contributor.authorWarren, Rob
dc.contributor.authorRasolofo, Voahangy
dc.contributor.authorMei, Jian
dc.contributor.authorGao, Qian
dc.contributor.authorGicquel, Brigitte
dc.date.accessioned2011-05-15T16:00:15Z
dc.date.available2011-05-15T16:00:15Z
dc.date.issued2011-01
dc.descriptionThe original publication is available at http:/www.plosone.orgen_ZA
dc.description.abstractBackground: The Beijing family is a successful group of M. tuberculosis strains, often associated with drug resistance and widely distributed throughout the world. Polymorphic genetic markers have been used to type particular M. tuberculosis strains. We recently identified a group of polymorphic DNA repair replication and recombination (3R) genes. It was shown that evolution of M. tuberculosis complex strains can be studied using 3R SNPs and a high-resolution tool for strain discrimination was developed. Here we investigated the genetic diversity and propose a phylogeny for Beijing strains by analyzing polymorphisms in 3R genes. Methodology/Principal Findings: A group of 3R genes was sequenced in a collection of Beijing strains from different geographic origins. Sequence analysis and comparison with the ones of non-Beijing strains identified several SNPs. These SNPs were used to type a larger collection of Beijing strains and allowed identification of 26 different sequence types for which a phylogeny was constructed. Phylogenetic relationships established by sequence types were in agreement with evolutionary pathways suggested by other genetic markers, such as Large Sequence Polymorphisms (LSPs). A recent Beijing genotype (Bmyc10), which included 60% of strains from distinct parts of the world, appeared to be predominant. Conclusions/Significance: We found SNPs in 3R genes associated with the Beijing family, which enabled discrimination of different groups and the proposal of a phylogeny. The Beijing family can be divided into different groups characterized by particular genetic polymorphisms that may reflect pathogenic features. These SNPs are new, potential genetic markers that may contribute to better understand the success of the Beijing family. © 2011 Mestre et al.en_ZA
dc.description.versionPublishers' Versionen_ZA
dc.format.extent7 p. : ill.
dc.identifier.citationMestre, O. et al. 2011. Phylogeny of Mycobacterium tuberculosis Beijing Strains Constructed from Polymorphisms in Genes Involved in DNA Replication, Recombination and Repair. PLoS ONE, 6(1): e16020, doi:10.1371/journal.pone.0016020.en_ZA
dc.identifier.issn1932-6203 (online)
dc.identifier.otherdoi:10.1371/journal.pone.0016020
dc.identifier.urihttp://hdl.handle.net/10019.1/11601
dc.publisherPublic Library of Science (PLOS)en_ZA
dc.rights.holderAuthors retain copyrighten_ZA
dc.subjectPolymorphic DNA repair replication and recombination (3R) genesen_ZA
dc.subjectPolymorphic genetic markersen_ZA
dc.subjectMycobacterium tuberculosisen_ZA
dc.subjectSingle nucleotide polymorphismen_ZA
dc.titlePhylogeny of Mycobacterium tuberculosis Beijing strains constructed from Polymorphisms in genes involved in DNA replication, recombination and repairen_ZA
dc.typeArticleen_ZA
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