Isolation of B-cells using Miltenyi MACS bead isolation kits
| dc.contributor.author | Moore, Dannielle K. | en_ZA |
| dc.contributor.author | Motaung, Bongani | en_ZA |
| dc.contributor.author | du Plessis, Nelita | en_ZA |
| dc.contributor.author | Shabangu, Ayanda N. | en_ZA |
| dc.contributor.author | Loxton, Andre G. | en_ZA |
| dc.contributor.author | SU-IRG Consortium | en_ZA |
| dc.date.accessioned | 2021-10-25T06:37:36Z | |
| dc.date.available | 2021-10-25T06:37:36Z | |
| dc.date.issued | 2019-03-20 | |
| dc.description | CITATION: Moore, D. K., et al. 2019. Isolation of B-cells using Miltenyi MACS bead isolation kits. PLoS ONE, 14(3). doi:10.1371/journal.pone.0213832 | |
| dc.description | The original publication is available at https://journals.plos.org/plosone/ | |
| dc.description.abstract | This article describes the procedures used to isolate pure B-cell populations from whole blood using various Miltenyi magnetic-activated cell sorting (MACS) bead Isolation kits. Such populations are vital for studies investigating the functional capacity of B-cells, as the presence of other cell types may have indirect effects on B-cell function through cell-cell interactions or by secretion of several soluble molecules. B-cells can be isolated by two main approaches: 1) Negative selection—in which B-cells remain “untouched” in their native state; this is advantageous as it is likely that B-cells remain functionally unaltered by this process. 2) Positive selection–in which B-cells are labelled and actively removed from the sample. We used three Negative B-cell isolation kits as well as the Positive B-cell isolation kit from Miltenyi and compared the purity of each of the resulting B-cells fractions. Contamination of isolated B-cell fractions with platelets was the conclusive finding for all of the isolation techniques tested. These results illustrate the inefficiency of current available MACS B-cell isolation kits to produce pure B-cell populations, from which concrete findings can be made. As such we suggest cell sorting as the preferred method for isolating pure B-cells to be used for downstream functional assays. | en_ZA |
| dc.description.uri | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0213832 | |
| dc.description.version | Publisher's version | |
| dc.format.extent | 15 pages | |
| dc.identifier.citation | Moore, D. K., et al. 2019. Isolation of B-cells using Miltenyi MACS bead isolation kits. PLoS ONE, 14(3). doi:10.1371/journal.pone.0213832 | |
| dc.identifier.issn | 1932-6203 (online) | |
| dc.identifier.other | doi:10.1371/journal.pone.0213832 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/123285 | |
| dc.language.iso | en_ZA | en_ZA |
| dc.publisher | Public Library of Science | |
| dc.rights.holder | Authors retain rights | |
| dc.subject | Miltenyi magnetic-activated cell sorting (MACS) | en_ZA |
| dc.subject.lcsh | Cell populations -- Separation | en_ZA |
| dc.subject.lcsh | B cells | en_ZA |
| dc.subject.lcsh | Separation (Technology) | en_ZA |
| dc.subject.lcsh | Molecular immunology | en_ZA |
| dc.title | Isolation of B-cells using Miltenyi MACS bead isolation kits | en_ZA |
| dc.type | Article | en_ZA |