The effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart : the involvement of the PI3-Kinase signaling pathway
| dc.contributor.author | Engelbrecht, Anna-Mart | |
| dc.contributor.author | Odendaal, Louise | |
| dc.contributor.author | Du Toit, Eugene F. | |
| dc.contributor.author | Kupai, Krisztina | |
| dc.contributor.author | Czont, Tamas | |
| dc.contributor.author | Ferdinandy, Peter | |
| dc.contributor.author | Van Rooyen, Jacques | |
| dc.date.accessioned | 2010-12-13T08:16:53Z | |
| dc.date.available | 2010-12-13T08:16:53Z | |
| dc.date.issued | 2009-05 | |
| dc.date.updated | 2010-11-02T13:08:30Z | |
| dc.description | Includes bibliography | |
| dc.description.abstract | We have previously shown that dietary red palm oil (RPO) supplementation improves functional recovery in hearts subjected to ischaemia/reperfusion-induced injury. Unfortunately, the cellular and molecular mechanisms responsible for this phenomenon are still poorly understood and no knowledge exists regarding the effects of RPO supplementation on the phosphoinositide 3-kinase (PI3-K) signaling pathway and apoptosis during ischaemia/reperfusion injury. Therefore, the aims of the present study were three fold: (i) to establish the effect of RPO on the functional recovery of the heart after ischaemia/reperfuion injury; (ii) to determine the effect of the PI3-K pathway in RPO-induced protection with the aid of an inhibitor (wortmannin); and (iii) to evaluate apoptosis in our model. Wistar rats were fed a standard rat chow control diet or a control diet plus 7 g RPO/kg for six weeks. Hearts were excised and mounted on a Langendorff perfusion apparatus. Mechanical function was measured after a 25 min period of total global ischaemia followed by 30 minutes of reperfusion. Hearts subjected to the same conditions were freeze-clamped for biochemical analysis at 10 min during reperfusion to determine the involvement of the PI3-Kinase signaling pathway and apoptosis in our model. Dietary RPO supplementation significantly increased % rate pressure product recovery during reperfusion (71.0 ± 6.3% in control vs 92.36 ± 4.489% in RPO; p < 0.05). The % rate pressure product recovery was significantly reduced when wortmannin was added during perfusion (92.36 ± 4.489% in the RPO group vs 75.21 ± 5.26% in RPO + Wm). RPO + Wm also significantly attenuated PI3-K induction compared with the RPO group (59.2 ± 2.8 pixels in RPO vs 37.9 ± 3.4 pixels in RPO + Wm). We have also demonstrated that PI3-K inhibition induced PARP cleavage (marker of apoptosis) in the hearts during ischaemia/reperfusion injury and that RPO supplementation counteracted this effect. | en_ZA |
| dc.description.version | Peer Reviewed | |
| dc.format.extent | 8 p. : ill. | |
| dc.identifier.citation | Engelbrecht, A-M, Odendaal, L, Du Toit, EF, Kupai, K, Csont, T, Ferdinandy, P & Van Rooyen 2009, 'The effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart: the involvement of the PI3-Kinase signaling pathway', Lipids in Health and Disease, 29;8(1):18. | en_ZA |
| dc.identifier.issn | 1476-511X | |
| dc.identifier.other | http://dx.doi.org/10.1186/1476-511X-8-18 | |
| dc.identifier.uri | http://hdl.handle.net/10019.1/5055 | |
| dc.language.iso | en_US | en_ZA |
| dc.language.rfc3066 | en | |
| dc.publisher | BioMed Central | en_ZA |
| dc.rights.holder | Engelbrecht et al.; licensee BioMed Central Ltd. | en_ZA |
| dc.subject | Dietary red palm oil (RPO) supplementation | en_ZA |
| dc.subject | Ischaemia/reperfusion injury | en_ZA |
| dc.subject | Heart recovery | en_ZA |
| dc.subject | PI3-K pathway in RPO-induced protection | en_ZA |
| dc.subject | Apoptosis | en_ZA |
| dc.subject.lcsh | Dietary supplement -- Experiments | en_ZA |
| dc.title | The effect of dietary red palm oil on the functional recovery of the ischaemic/reperfused isolated rat heart : the involvement of the PI3-Kinase signaling pathway | en_ZA |
| dc.type | Article | en_ZA |